Product Pathways - Cell Cycle / Checkpoint
Cyclin Antibody Sampler Kit #9869
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Cyclin A2 (BF683) Mouse mAb #4656 | 40 µl | W F | H | 55 | Mouse IgG2b |
| Cyclin B1 Antibody #4138 | 40 µl | W IF-IC | H M R Hm Mk | 58 | Rabbit |
| Cyclin D1 (92G2) Rabbit mAb #2978 | 40 µl | W IHC-P F | H M R | 36 | Rabbit IgG |
| Cyclin D2 (D52F9) Rabbit mAb #3741 | 40 µl | W IP | H M R | 31 | Rabbit IgG |
| Cyclin D3 (DCS22) Mouse mAb #2936 | 40 µl | W IHC-P | H M R | 31 | Mouse IgG1 |
| Cyclin E1 (HE12) Mouse mAb #4129 | 40 µl | W | H M R Hm Mk | 48-56 | Mouse IgG1 |
| Cyclin E2 Antibody #4132 | 40 µl | W | H | 48 | Rabbit |
| Cyclin H Antibody #2927 | 40 µl | W IP | H M R | 36 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| Anti-mouse IgG, HRP-linked Antibody #7076 | 100 µl | Horse |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the Cyclin Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other cyclin proteins.
Western Blotting
Western blot analysis of extracts from Jurkat and Raji cells using Cyclin H Antibody #2927.
Western Blotting
Western blot analysis of extracts from SK-N-MC, C6 and mIMCD-3 cells using Cyclin D3 (DCS22) Mouse mAb #2936.
Western Blotting
Western blot analysis of extracts from MCF7, L929 and C6 cells using Cyclin D1 (92G2) Rabbit mAb #2978.
Western Blotting
Western blot analysis of extracts from various cell lines using Cyclin E (HE12) Mouse mAb #4129.
Western Blotting
Western blot analysis of extracts from MCF-7, SK-N-MC and HeLa cells, untreated or treated with the proteasome inhibitor MG-132, using Cyclin E2 Antibody #4132.
Western Blotting
Western blot analysis of extracts from various cell lines using Cyclin B1 Antibody #4138.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or treated with either doxorubicin (0.5 µM, 24 hours) or nocodazole (50 ng/ml, 24 hours), using Cyclin A (BF683) Mouse mAb #4656.
Western blot analysis of extracts from various cell lines using Cyclin D2 (D52F9) XP® Rabbit mAb #3741.
Description
The Cyclin Antibody Sampler Kit provides an economical means of evaluating the presence of cyclin proteins in cells. The kit contains enough primary and secondary antibodies to perform four western blot experiments with each antibody.
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues within the carboxy terminus of cyclin D1, the amino terminus of cyclin D2, recombinant human cyclin E1, or cyclin A2. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues within the carboxy terminus of cyclin H, cyclin E2, or the amino terminus of cyclin B. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Background
Control of the cell cycle is regulated by a multitude of cellular events and processes. The cyclin dependent kinases (CDK) regulate many of these pathways and constitute an active complex when associated with their cyclin partners. This activity is controlled primarily by phosphorylation, which determines subcellular localization of the CDK/cyclin complex (1,2). Some phosphorylation events control the function of cytoplasmic retention sequences while other events regulate nuclear localization and export sequence function (3,4). Cyclin and cyclin-dependent kinase inhibitor (CKI) levels are regulated by ubiquitination and degradation via the ubiquitin proteasome pathway (5). A variety of CKI proteins associate with these complexes and modulate access to regulatory domains on cyclins (6). Additional complexity is generated as the controlled protein levels of each cyclin oscillate with the stages of cell cycle. Increased expression of Cyclin D1 is associated with certain types of cancer (7,8) and may associate with TSC2 (tuberin) independent of its Cdk partner (9).
- Krempler, A. et al. (2005) Cell Mol Life Sci 62, 1379-87.
- Toyoshima, F. et al. (1998) EMBO J 17, 2728-35.
- Porter, L.A. and Donoghue, D.J. (2003) Prog Cell Cycle Res 5, 335-47.
- Benzeno, S. et al. (2006) Oncogene 25, 6291-303.
- Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.
- Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
- Sherr, C.J. (1996) Science 274, 1672-7.
- Kim, J.K. and Diehl, J.A. (2009) J Cell Physiol 220, 292-6.
- Zacharek, S.J. et al. (2005) Cancer Res 65, 11354-60.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Protocols
- 2927:
- Immunoprecipitation, Western Blotting
- 2936:
- IHC / Paraffin, Western Blotting
- 2978:
- Flow, IHC / Paraffin, Western Blotting
- 3741:
- Immunoprecipitation, Western Blotting
- 4129:
- Western Blotting
- 4132:
- Western Blotting
- 4138:
- Immunofluorescence, Western Blotting
- 4656:
- Flow, Western Blotting
Companion Products
- 7727 Biotinylated Protein Ladder Detection Pack
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 9868 CDK Antibody Sampler Kit
For Research Use Only. Not For Use In Diagnostic Procedures.
