Product Pathways - Metabolism
O-GlcNAc (CTD110.6) Mouse mAb #9875
|9875S||100 µl (10 western blots)||---||In Stock||---|
|9875||carrier free and custom formulation / quantity||email request|
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|W||1:1000||All Species Expected||Endogenous||Mouse IgM|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
O-GlcNAc (CTD110.6) Mouse mAb specifically recognizes endogenous levels of O-GlcNAc on proteins in β-O-glycosidic linkage to both serine and threonine.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a peptide containing serine-O-linked N-acetylglucosamine (O-GlcNAc).
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with PUGNAc (+), an inhibitor of N-acetyl-β-D-glucosaminidase, using O-GlcNAc (CTD110.6) Mouse mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).
O-GlcNAcylation is a post-translational modification where N-acetyl-D-glucosamine (GlcNAc) is covalently linked to cytoplasmic and nuclear proteins at serine or threonine residues (1,2). This modification is important in many cellular processes including metabolism, cell growth and morphogenesis, apoptosis and transcription (2,3) and has been implicated to play a critical role in cancer (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at firstname.lastname@example.org.