Cell Signaling Technology

Product Pathways - Metabolism

O-GlcNAc (CTD110.6) Mouse mAb #9875

Applications Reactivity Sensitivity Isotype
W All Endogenous Mouse IgM

Applications Key:  W=Western Blotting
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

Specificity / Sensitivity

O-GlcNAc (CTD110.6) Mouse mAb specifically recognizes endogenous levels of O-GlcNAc on proteins in β-O-glycosidic linkage to both serine and threonine.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a peptide containing serine-O-linked N-acetylglucosamine (O-GlcNAc).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells in the absence or presence of PUGNAC, an inhibitor of N-acetyl-β-d-glucosaminidase, using O-GlcNAc (CTD110.6) Mouse mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using O-GlcNAc (CTD110.6) Mouse mAb.

Background

O-GlcNAcylation is a post-translational modification where β-D-N-acetylglucosamine (GlcNAc) is covalently linked to cytoplasmic and nuclear proteins at serine or threonine residues (1,2). This modification is important in many cellular processes including metabolism, cell growth and morphogenesis, apoptosis and transcription (2,3) and has been implicated to play a critical role in cancer (1).

  1. Comer, F.I. et al. (2001) Anal Biochem 293, 169-77.
  2. Slawson, C. and Hart, G.W. (2011) Nat Rev Cancer 11, 678-84.
  3. Capotosti, F. et al. (2011) Cell 144, 376-88.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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