Cell Signaling Technology

Product Pathways - Metabolism

IRAP (3E1) Mouse mAb #9876

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC M (R) Endogenous 165 Mouse IgG1

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IRAP (3E1) Mouse mAb recognizes endogenous levels of total IRAP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a fusion protein corresponding to the amino terminus of rat IRAP.

Western Blotting

Western Blotting

Western blot analysis of extracts from 3T3-L1 cells using IRAP (3E1) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of differentiated 3T3-L1 cells, treated with LY294002 #9901 (50 μM, 2hr; left) or insulin (100 nM, 30 min; right), using IRAP (3E1) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

IRAP (also known as LNPEP) was originally described as an insulin-responsive aminopeptidase found in Glut4-containing vesicles (1). It is essentially always in the same compartments as Glut4 and has identical insulin-stimulated translocation patterns as Glut4 (2). IRAP is therefore considered to be a surrogate marker for Glut4 (2). IRAP was later found to be a critical enzyme that regulates the expression and activity of several essential hormones and regulatory proteins, including the Glut4 transporter (3,4). This membrane associated, zinc-dependent cystinyl aminopeptidase acts as both a receptor for angiotensin IV as well as the enzyme that catalyzes the synthesis of this essential hormone from its angiotensinogen precursor (5). IRAP catalyzes the hydrolysis of several peptide hormones, including oxytocin and vasopressin (4). Abnormal IRAP expression or activity is associated with several forms of cancer in humans, including renal and endometrial cancers (6,7).

  1. Garza, L.A. and Birnbaum, M.J. (2000) J Biol Chem 275, 2560-7.
  2. Gross, D.N. et al. (2004) Mol Cell Biol 24, 7151-62.
  3. Albiston, A.L. et al. (2001) J Biol Chem 276, 48623-6.
  4. Keller, S.R. (2003) Front Biosci 8, s410-20.
  5. Vanderheyden, P.M. (2009) Mol Cell Endocrinol 302, 159-66.
  6. Larrinaga, G. et al. (2007) Regul Pept 144, 56-61.
  7. Suzuki, Y. et al. (2003) Clin Cancer Res 9, 1528-34.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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