Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Roscovitine #9885

Molecular Formula:
C19H28N6O
Molecular Weight:
354.46 g/mol
Purity:
>99%

Directions for Use

Roscovitine [(R) stereoisomer] is supplied as a 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 143 μl DMSO or MeOH to make a 20 mM stock solution. The suggested working concentration is 20 μM in tissue culture medium. Treat cells for 4-24 hours. Store solution at -20ºC.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells treated with Roscovitine (20 μM) for the indicated times using Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb #2325 (left) or Phospho-(Ser) CDKs Substrate Antibody #2324 (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells treated with Roscovitine (20 μM) for the indicated times using an antibody to Rb.

Structure

Structure

Structure of Roscovitine


Background

Roscovitine is a cell permeable reversible selective inhibitor of cyclin-dependent kinases CDK1 (cdc2), CDK2 and CDK5 (1). A purine analog, this drug competes for the binding site of ATP in the catalytic cleft. Treatment of cultured cells with roscovitine can cause cell cycle arrest or apoptosis (1-4). The IC50 for cdc2 activity is 0.65 μM in vitro (1).

  1. Meijer, L. et al. (1997) Eur J Biochem 243, 527-36.
  2. Whittaker, S.R. et al. (2007) Cell Cycle 6, 3114-31.
  3. Dey, A. et al. (2008) Cell Death Differ 15, 263-73.
  4. Wojciechowski, J. et al. (2003) Int J Cancer 106, 486-95.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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