Cell Signaling Technology

Product Pathways - Apoptosis

NBR1 (D2E6) Rabbit mAb #9891

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H M Endogenous 120 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

NBR1 (D2E6) Rabbit mAb recognizes endogenous levels of total NBR1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys601 of human NBR1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NBR1 (D2E6) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF7 cells using NBR1 (D2E6) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with bafilomycin A (0.2 μM, 18 hr; right), using NBR1 (D2E6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

Next to BRCA1 gene 1 (NBR1) protein is known for its encoding gene proximity to the BRCA1 tumor suppressor gene (1,2). N-terminal Phox and Bem1p (PB1) domains of NBR1 mediate its interaction with muscle specific titin kinase (3,4) and scaffolding protein p62 (4). NBR1 plays a role in autophagy by facilitating the autophagosomal degradation of ubiquitinated proteins independently and also in concert with p62 (5,6).

  1. Campbell, I.G. et al. (1994) Hum Mol Genet 3, 589-94.
  2. Miki, Y. et al. (1994) Science 266, 66-71.
  3. Lange, S. et al. (2005) Science 308, 1599-603.
  4. Müller, S. et al. (2006) FEBS Lett 580, 341-4.
  5. Kirkin, V. et al. (2009) Mol Cell 33, 505-16.
  6. Lamark, T. et al. (2009) Cell Cycle 8, 1986-90.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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