Product Pathways - Lymphocyte Signaling
β2-microglobulin Antibody #9899
|9899S||100 µl (10 western blots)||---||In Stock||---|
|9899||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
β2-microglobulin Antibody recognizes endogenous levels of total β2-microglobulin (B2M) protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro52 of human β2-microglobulin (B2M) protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® β2-microglobulin siRNA I #7759 (+), or SignalSilence® β2-microglobulin siRNA II #7774 (+), using β2-microglobulin Antibody (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The β2-microglobulin Antibody confirms silencing of β2-microglobulin expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control.
β2-microglobulin (B2M) is a principal component of the Major Histocompatibility Complex (MHC) class I molecule, a ternary membrane protein complex that displays fragments derived from proteolyzed cytosolic proteins on the surface of cells for recognition by the surveillance immune system (1,2). As an integral component of the MHC class I complex, β2-microglobulin plays a critically important role in immune system function (3). It has important relevance to cancer biology research; for example, research studies have shown that nearly one-third of diffuse large B cell lymphomas contain mutations that inactivate β2-microglobulin gene function, thereby allowing tumor cells to escape immune detection (4). In addition, β2-microglobulin has been identified as an amyloid preprotein with collagen-binding affinity (5); its accumulation in osteoarthritic lesions of long-term dialysis patients is reportedly a contributing factor to the condition known as amyloid osteoarthropathy (6).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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