Product Pathways - Tyrosine Kinase/ Adaptors
Human Stem Cell Factor (SCF) #9907
| MW | Source | Purity |
|---|---|---|
| 18.5 kDa | Human Recombinant Protein |
Description
The human SCF coding cDNA was obtained from human bone marrow stromal cell mRNA, subcloned into a prokaryotic expression vector and expressed in E. coli. The recombinant human SCF protein was purified and stored in PBS buffer containing 0.1% BSA.
Concentration and Specific Activity
100 µg/ml
Western Blotting
Western blot analysis of extracts from MO7e cells, untreated or SCF-stimulated (50 ng/ml for 2 minutes), using Phospho-c-Kit (Tyr719) Antibody #3391 (upper) or c-Kit Antibody #3392 (lower).
Directions for Use
CST recommends using 50-100 ng/ml of SCF for stimulation of SCF signaling.
Background
Stem cell factor (SCF) is a growth factor that is essential for haemopoiesis, melanogenesis and fertility. SCF is also known as mast cell growth factor (MCGF), steel factor (SLF), or kit ligand (KL) (1-3). SCF mediates its biological effects by binding to and activating c-Kit (4). SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates, and activation of multiple signaling pathways (5,6). SCF/c-Kit may take part in the growth control of human malignancies (7).
- Smith, M.A. et al. (2001) Blood Rev. 15, 191-197.
- Linnekin, D. (1999) Int. J. Biochem. Cell Biol. 31, 1053-1074.
- Huang, E. et al. (1990) Cell 63, 225-233.
- Jiang, X. et al. (2000) EMBO J. 19, 3192-3203.
- Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31, 1037-1051.
- Ueda, S. et al. (2002) Blood 99, 3342-3349.
- Attoub, S. et al. (2002) Cancer Res. 62, 4879-4883.
Application References
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