Product Pathways - MAPK Signaling
Phospho-MAPK Family Antibody Sampler Kit #9910
|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511||40 µl||W IP IHC-P IF-IC F||H M R Mk Mi Pg Sc (Hm) (C) (Z) (B)||43||Rabbit IgG|
|Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370||40 µl||W IP IHC-P IF-IC F||H M R Hm Mk Mi Dm Z B Dg Pg Sc (C) (Ce)||44, 42||Rabbit IgG|
|Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668||40 µl||W IP IHC-P||H M R Dm Sc||46, 54||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|U0126 #9903||0.3 milligrams||380.50|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Mi=Mink Dm=D. melanogaster Z=Zebrafish B=Bovine Dg=Dog Pg=Pig Sc=S. cerevisiae Ce=C. elegans
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-p44/42 MAPK, Phospho-SAPK/JNK and Phospho-p38 MAPK Antibodies only recognize the phosphorylated forms of p44/42 MAPK, SAPK/JNK and p38 MAPK, respectively. They do not significantly cross-react with other MAPK family members.
Western blot analysis of extracts from 293, NIH/3T3 and C6 cells, treated with λ phosphatase or TPA as indicated, using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper), or p44/42 MAP Kinase (137F5) Rabbit mAb #4695 (lower).
Western blot analysis of extracts from COS and 293 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (upper) or p38 MAPK Antibody #9212 (lower).
Western blot analysis of extracts from 293 cells, untreated or UV-treated, NIH/3T3 cells, untreated or UV-treated and C6 cells, untreated or anisomycin-treated, using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668.
The Phospho-MAPK Family Antibody Sampler Kit provides an economical means of evaluating the phosphorylation state of p38, p44/42, and SAPK/JNK mitogen-activated protein kinases. The kit contains enough primary and secondary antibodies to perform four western blot experiments.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides surrounding Thr180/Tyr182 of human p38 MAPK, Thr202/Tyr204 of human p44 MAPK or Thr183/Tyr185 of human SAPK/JNK.
p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).
- Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.
- Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
- Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
- Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
- Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.
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* Product-specific protocol.
- 9926 MAPK Family Antibody Sampler Kit
- 9900 PD98059
- 9903 U0126
- 4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
- 9107 p44/42 MAPK (Erk1/2) (3A7) Mouse mAb
- 9212 p38 MAPK Antibody
- 9228 p38α MAPK (L53F8) Mouse mAb
- 9252 SAPK/JNK Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.