Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MAPK Family Antibody Sampler Kit #9910

Kit Includes Quantity Applications Reactivity MW (kDa) Source
Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb # 4370 40 microliters W IP IHC-P IF-IC F H M R Mk Mi Hm B Dr Z 44, 42 kDa Rabbit
Phospho-p38 MAPK (Thr180/Tyr182) Antibody # 9211 40 microliters W IP IF-IC F H M R Mk Dr 43 Rabbit
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb # 4668 40 microliters W IP IHC-P H M R Dr 46, 54 Rabbit
Anti-rabbit IgG, HRP-linked Antibody # 7074 50 microliters Goat
U0126 (MEK1/2 Inhibitor) # 9903 0.3 milligrams

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Mi=Mink  Hm=Hamster  B=Bovine  Dr=Drosophila  Z=Zebra Fish

Specificity / Sensitivity

Phospho-p44/42 MAPK, Phospho-SAPK/JNK and Phospho-p38 MAPK Antibodies only recognize the phosphorylated forms of p44/42 MAPK, SAPK/JNK and p38 MAPK, respectively. They do not significantly cross-react with other MAPK family members.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293, NIH/3T3 and C6 cells, treated with λ phosphatase or TPA as indicated, using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370 (upper), or p44/42 MAP Kinase (137F5) Rabbit mAb #4695 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or UV-treated, NIH/3T3 cells, untreated or UV-treated and C6 cells, untreated or anisomycin-treated, using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or UV-treated, using Phospho-p38 MAP Kinase (Thr180/Tyr182) Antibody #9211 or p38 MAP Kinase Antibody #9212.


Source / Purification

Polyclonal antibody is produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Thr180/Tyr182 of human p38 MAP kinase, and purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing rabbits with synthetic phosphopeptides (KLH-coupled) surrounding Thr202/Tyr204 of human p44 MAP kinase or Thr183/Tyr185 of human SAPK/JNK.For MEK1/2 Inhibitor (U0126) #9903 information, see page 2.

Background

p44/42 MAPK (Erk1 and Erk2), SAPK/JNK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation and control of cellular responses to cytokines and stress.p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK).SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase.

  1. Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.
  2. Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
  3. Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
  4. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
  5. Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Product Pathways

Drug Discovery Tools

Featured Technologies

Protein Classes