Product Pathways - MAPK Signaling

Phospho-MAPK Family Antibody Sampler Kit #9910

Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Isotype
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP^® Rabbit mAb #4511	40 µl	W IP IHC-P IF-IC F	H M R Mk Sc (Hm) (C) (Z) (B) (Pg)	43	Rabbit IgG
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP^® Rabbit mAb #4370	40 µl	W IP IHC-P IF-IC F	H M R Hm Mk Mi Dm Z B Dg Pg Sc (Ce)	44, 42	Rabbit IgG
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668	40 µl	W IP IHC-P	H M R Dm Sc	46, 54	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074	100 µl				Goat
U0126 (MEK1/2 Inhibitor) #9903	0.3 milligrams

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Mi=Mink Dm=D. melanogaster Z=Zebrafish B=Bovine Dg=Dog Pg=Pig Sc=S. cerevisiae Ce=C. elegans
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Phospho-p44/42 MAPK, Phospho-SAPK/JNK and Phospho-p38 MAPK Antibodies only recognize the phosphorylated forms of p44/42 MAPK, SAPK/JNK and p38 MAPK, respectively. They do not significantly cross-react with other MAPK family members.

Western Blotting

Western blot analysis of extracts from 293, NIH/3T3 and C6 cells, treated with λ phosphatase or TPA as indicated, using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) XP^® Rabbit mAb #4370 (upper), or p44/42 MAP Kinase (137F5) Rabbit mAb #4695 (lower).

Western Blotting

Western blot analysis of extracts from COS and 293 cells, untreated or UV-treated, using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP^® Rabbit mAb #4511 (upper) or p38 MAPK Antibody #9212 (lower).

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or UV-treated, NIH/3T3 cells, untreated or UV-treated and C6 cells, untreated or anisomycin-treated, using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668.

Description

The Phospho-MAPK Family Antibody Sampler Kit provides an economical means of evaluating the phosphorylation state of p38, p44/42, and SAPK/JNK mitogen-activated protein kinases. The kit contains enough primary and secondary antibodies to perform four western blot experiments.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides surrounding Thr180/Tyr182 of human p38 MAPK, Thr202/Tyr204 of human p44 MAPK or Thr183/Tyr185 of human SAPK/JNK.

Background

p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).

Application References

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Protocols

4370:: Flow, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
4511:: Flow, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
4668:: IHC / Paraffin, Immunoprecipitation, Western Blotting

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.