Cell Signaling Technology

Product Pathways - MAPK Signaling

MAPK Family Antibody Sampler Kit #9926

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 40 µl W IP IHC-P IF-IC F H M R Hm Mk Mi Dm Z B Dg Pg Ce (C) 42, 44 Rabbit IgG
SAPK/JNK (56G8) Rabbit mAb #9258 40 µl W H M R Hm Mk Mi 46, 54 Rabbit IgG
p38 MAPK (D13E1) XP® Rabbit mAb #8690 40 µl W IHC-P IF-IC F H M R Hm Mk Pg (C) 40 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  Mi=Mink  Dm=D. melanogaster  Z=Zebrafish  B=Bovine  Dg=Dog  Pg=Pig  Ce=C. elegans
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the MAPK Family Antibody Sampler Kit recognizes only its specific target. The antibodies do not cross-react with other MAP kinases.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p44/42 MAP Kinase (137F5) Rabbit mAb #4695.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using p38 MAPK (D13E1) XP® Rabbit mAb #8690.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells using SAPK/JNK (56G8) Rabbit mAb #9258.


Description

The MAPK Family Antibody Sampler Kit provides an economical means of evaluating total levels of p38, p44/42, and SAPK/JNK mitogen-activated protein kinases. The kit contains enough primary and secondary antibody to perform four western blot experiments.

Source / Purification

p44/42 MAP Kinase (137F5) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase. SAPK/JNK (56G8) Rabbit mAb is produced by immunizing animals with a human JNK2/MBP fusion protein. p38 MAPK (D13E1) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human p38 protein.

Background

p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).

  1. Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.
  2. Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
  3. Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
  4. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
  5. Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.

Application References

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