Cell Signaling Technology

Product Pathways - MAPK Signaling

MAPK Family Antibody Sampler Kit #9926

Kit Includes Quantity Applications Reactivity MW (kDa) Source
p44/42 MAP Kinase (137F5) Rabbit mAb # 4695 40 microliters W IP IHC-P IF-IC F H M R Mk Mi Hm B Dr Z 42, 44 Rabbit
SAPK/JNK (56G8) Rabbit mAb # 9258 40 microliters W H M R Mk Mi Hm 46, 54 Rabbit
p38 MAP Kinase Antibody # 9212 40 microliters W IHC-P IF-IC F H M R Mk 43 Rabbit
Anti-rabbit IgG, HRP-linked Antibody # 7074 50 microliters Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Mi=Mink  Hm=Hamster  B=Bovine  Dr=Drosophila  Z=Zebra Fish

Specificity / Sensitivity

Each antibody in the MAPK Family Antibody Sampler Kit recognizes only its specific target. The antibodies do not cross-react with other MAP kinases.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p44/42 MAP Kinase (137F5) Rabbit mAb #4695.

Western Blotting

Western Blotting

Western blot analysis of extracts from UV-treated NIH/3T3 cells, using Phospho-p38 MAP Kinase (Thr180/Tyr182) Antibody #9211 (upper) or control p38 MAP Kinase Antibody #9212 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells using SAPK/JNK (56G8) Rabbit mAb #9258.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAP Kinase Antibody #9212.

IHC-P (paraffin)

IHC-P (paraffin)

mmunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAP Kinase Antibody #9212.

Source / Purification

p44/42 MAP Kinase Antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the C-terminus of rat p44 MAP kinase. SAPK/JNK (56G8) Rabbit mAb is produced by immunizing rabbits with a human JNK2/MBP fusion protein. p38 MAP Kinase Antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human p38 MAP kinase. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

p44/42 MAPK (Erk1 and Erk2), SAPK/JNK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation and control of cellular responses to cytokines and stress.p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK).SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase.

  1. Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.
  2. Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
  3. Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
  4. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
  5. Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.

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