Product Pathways - MAPK Signaling

MAPK Family Antibody Sampler Kit #9926

Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Isotype
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695	40 µl	W IP IHC-P IF-IC F	H M R Hm Mk Mi Dm Z B Dg Pg Ce (C)	42, 44	Rabbit IgG
SAPK/JNK (56G8) Rabbit mAb #9258	40 µl	W	H M R Hm Mk Mi	46, 54	Rabbit IgG
p38 MAPK Antibody #9212	40 µl	W IHC-P IF-IC F	H M R Mk GP (C)	43	Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074	100 µl				Goat

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Mi=Mink Dm=D. melanogaster Z=Zebrafish B=Bovine Dg=Dog Pg=Pig GP=Guinea Pig Ce=C. elegans
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the MAPK Family Antibody Sampler Kit recognizes only its specific target. The antibodies do not cross-react with other MAP kinases.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p44/42 MAP Kinase (137F5) Rabbit mAb #4695.

Western Blotting

Western blot analysis of extracts from UV-treated NIH/3T3 cells, using Phospho-p38 MAP Kinase (Thr180/Tyr182) Antibody #9211 (upper) or control p38 MAP Kinase Antibody #9212 (lower).

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells using SAPK/JNK (56G8) Rabbit mAb #9258.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAP Kinase Antibody #9212.

IHC-P (paraffin)

mmunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using p38 MAP Kinase Antibody #9212.

Description

The MAPK Family Antibody Sampler Kit provides an economical means of evaluating total levels of p38, p44/42, and SAPK/JNK mitogen-activated protein kinases. The kit contains enough primary and secondary antibody to perform four western blot experiments.

Source / Purification

p44/42 MAP Kinase Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase. SAPK/JNK (56G8) Rabbit mAb is produced by immunizing animals with a human JNK2/MBP fusion protein. p38 MAP Kinase Antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human p38 MAP kinase. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5).

Application References

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Protocols

4695:: Flow, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
9212:: Flow, IHC / Paraffin, Immunofluorescence, Western Blotting
9258:: Western Blotting

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.