Product Pathways - Chromatin Regulation
Acetyl-Histone H3 Antibody Sampler Kit #9927
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Acetyl-Histone H3 (Lys9) Antibody # 9671 | 40 microliters | W IP IHC-P ChIP | H M R Mk Dm Sc | 17 | Rabbit |
| Phospho-Histone H3 (Ser10) Antibody # 9701 | 40 microliters | W IP IHC-P IHC-F IF-IC F | H M R Mk C Dm Z Sc (X) | 17 | Rabbit |
| Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody # 9711 | 40 microliters | W IHC-P IF-P | H M R | 17 | Rabbit |
| Acetyl-Histone H3 (Lys18) Antibody # 9675 | 40 microliters | W IHC-P ChIP | H M R | 17 | Rabbit |
| Acetyl-Histone H3 (Lys23) Antibody # 9674 | 40 microliters | W IHC-P | H M R | 17 | Rabbit |
| Histone H3 Antibody # 9715 | 40 microliters | W IHC-P IF-IC | H M R Mk Z B Pg (Dm) | 17 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 100 microliters | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
IF-P=Immunofluorescence (Paraffin)
F=Flow Cytometry
ChIP=Chromatin IP
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
C=Chicken
Dm=D. melanogaster
X=Xenopus
Z=Zebrafish
B=Bovine
Pg=Pig
Sc=S. cerevisiae
Specificity / Sensitivity
All antibodies in the Acetyl-Histone H3 Antibody Sampler Kit recognize histone H3 only when modified at the indicated site.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated, TSA-treated to induce histone acetylation, or treated with serum plus calyculin A to induce H3 phosphorylation, or both, using Acetyl-Histone H3 (Lys9) Antibody #9671.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or TSA-treated (400 nM for 12 hours), using Acetyl-Histone H3 (Lys23) Antibody #9674 (lanes 1-2), Histone H3 Antibody #9712 (lanes 3-4); or Acetyl-Histone H3 (Lys23) Antibody pretreated with specific nonacetyl peptide (lanes 5-6) or pretreated with specific acetyl-H3 peptide (lanes 7-8).
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells with or without TSA treatment, using Acetyl-Histone H3 (Lys18) Antibody #9675.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated, TSA-treated to induce acetylation, treated with serum plus calyculin A to induce phosphorylation of H3, or both, using Phospho-Histone H3 (Ser10) Antibody #9701.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated, TSA-treated to induce histone acetylation, treated wih serum plus calyculin A to induce phosphorylation of H3, or both, using Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody #9711.
Western Blotting
Western blot analysis of extracts from various cell lines, using Histone H3 Antibody #9715.
Description
Description: The Acetyl- Histone H3 Antibody Sampler Kit provides a fast and economical means of evaluating the phosphorylation and acetylation sites on Histone H3. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with synthetic phosphorylated or acetylated peptides corresponding to residues surrounding Lys9, Ser10, Lys18 or Lys23 of human Histone H3. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27 and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11).
- Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
- Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
- Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
- Cheung, P. et al. (2000) Cell 103, 263-71.
- Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
- Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
- Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
- Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
- Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
- Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
- Dai, J. et al. (2005) Genes Dev 19, 472-88.
Application References
- Allison, S.J. and Milner, J. (2003) Loss of p53 has site-specific effects on histone H3 modification, including serine 10 phosphorylation important for maintenance of ploidy. Cancer Res. 63, 6674-6679. Applications: Western Blotting
- Parsons, X. H. et al. (2003) Histone deacetylation by Sir2 generates a transcriptionally repressed nucleoprotein complex. Proc. Nat. Acad. Sci. USA 100, 1609-1614. Applications: Western Blotting
- Yoon, H. G. et al. (2003) Purification and functional characterization of the human N-CoR complex: the roles of HDAC3, TBL1 and TBLR1. EMBO J. 22, 1336-1346. Applications: Western Blotting
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Protocols
- 9671:
- ChIP Agarose, ChIP Magnetic, IHC / Paraffin, Immunoprecipitation, Western Blotting
- 9674:
- IHC / Paraffin, Western Blotting
- 9675:
- IHC / Paraffin, Immunoprecipitation, Western Blotting
- 9701:
- Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
- 9711:
- IHC / Paraffin, Immunofluorescence, Western Blotting
- 9715:
- IHC / Paraffin, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
Companion Products
- 9928 Histone Deacetylase (HDAC) Antibody Sampler Kit
- 2252 PRMT5/Skb1Hs Methyltransferase Antibody
- 2991 SUV39H1 Histone Methyltransferase Antibody
- 2152 WSTF Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
