Product Pathways - Chromatin Regulation

Acetyl-Histone H3 Antibody Sampler Kit #9927

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Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Source
Acetyl-Histone H3 (Lys9) Antibody # 9671	40 microliters	W IP IHC-P IC	H M R	17	Rabbit
Phospho-Histone H3 (Ser10) Antibody # 9701	40 microliters	W IP IHC-P IHC-F IF-IC F	H M R Mk C (X)	17	Rabbit
Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody # 9711	40 microliters	W IHC-P IF-P IC	H M R	17	Rabbit
Acetyl-Histone H3 (Lys18) Antibody # 9675	40 microliters	W IHC-P	H M R	17	Rabbit
Acetyl-Histone H3 (Lys23) Antibody # 9674	40 microliters	W IHC-P	H M R	17	Rabbit
Histone H3 Antibody # 9715	40 microliters	W IP IHC-P IF-IC	H M R Mk B (Dr)	17	Rabbit
Anti-rabbit IgG, HRP-linked Antibody # 7074	100 microliters				Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  IF-P=Immunofluorescence (Paraffin)  IC=Immunocytochemistry  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  B=Bovine  Dr=Drosophila  X=Xenopus

Specificity / Sensitivity

All antibodies in the Acetyl-Histone H3 Antibody Sampler Kit recognize histone H3 only when modified at the indicated site.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with synthetic phosphorylated or acetylated peptides (KLH-coupled) corresponding to residues surrounding Lys9, Ser10, Lys18 or Lys23 of human Histone H3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23 (2,3). Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11).

1. Workman, J.L. and Kingston, R.E. (1998) Annu. Rev. Biochem. 67, 545-579.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-17641.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-45.
4. Cheung, P. et al. (2000) Cell 103, 263-271.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem. Biol. 9, 1167-1173.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat. Cell Biol. 5, 395-399.
7. Thorne, A.W. et al. (1990) Eur. J. Biochem. 193, 701-713.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-360.
9. Goto, H. et al. (1999) J. Biol. Chem. 274, 25543-25549.
10. Preuss, U. et al. (2003) Nucleic Acids Res. 31, 878-885.
11. Dai, J. et al. (2005) Genes Dev. 19, 472-488.

Application References

• Allison, S.J. and Milner, J. (2003) Loss of p53 has site-specific effects on histone H3 modification, including serine 10 phosphorylation important for maintenance of ploidy. Cancer Res. 63, 6674-6679. This article references the use of Acetyl-Histone H3 Antibody Sampler Kit in the following applications: Western Blotting
• Parsons, X. H. et al. (2003) Histone deacetylation by Sir2 generates a transcriptionally repressed nucleoprotein complex. Proc. Nat. Acad. Sci. USA 100, 1609-1614. This article references the use of Acetyl-Histone H3 Antibody Sampler Kit in the following applications: Western Blotting
• Yoon, H. G. et al. (2003) Purification and functional characterization of the human N-CoR complex: the roles of HDAC3, TBL1 and TBLR1. EMBO J. 22, 1336-1346. This article references the use of Acetyl-Histone H3 Antibody Sampler Kit in the following applications: Western Blotting

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