Product Pathways - Chromatin Regulation / Epigenetics
Histone Deacetylase (HDAC) Antibody Sampler Kit #9928
|9928S||1 Kit (6 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|HDAC1 (10E2) Mouse mAb #5356||40 µl||W, IP, IF-IC||H, M, R, Mk||62||Mouse IgG1|
|HDAC2 (3F3) Mouse mAb #5113||40 µl||W, IP, IF-IC||H, M, R, Mk||60||Mouse IgG1|
|HDAC3 (7G6C5) Mouse mAb #3949||40 µl||W, IP, IF-IC||H, M, R, Mk||49||Mouse IgG2a|
|HDAC4 (D15C3) Rabbit mAb #7628||40 µl||W, IP||H, M, R, Mk||140||Rabbit IgG|
|Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb #3443||40 µl||W, IP||H, M||140, 124, 120||Rabbit IgG|
|HDAC6 (D2E5) Rabbit mAb #7558||40 µl||W, IP, IHC-P, IF-IC||H, Mk||160||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Anti-mouse IgG, HRP-linked Antibody #7076||100 µl||Horse|
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Western blot analysis of extracts from DO11.10 thymocyte hybridoma cells, either untreated or treated for 1 h with TPA (0.2 uM) and ionomycin (0.33 uM), using Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb #3443. Phospho-specificity of the antibody was determined by treating cell extracts with lambda phosphatase. Total HDAC proteins were detected using Histone Deacetylase 4 (HDAC4) Antibody #2072, Histone Deacetylase 5 (HDAC5) Antibody #2082 and Histone Deacetylase 7 (HDAC7) Antibody #2882.
Western blot analysis of extracts from various cell lines using HDAC3 (7G6C5) Mouse mAb #3949.
Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb #5113.
Western blot analysis of extracts from HeLa, NIH/3T3, and H-4-II-E cells using HDAC1 (10E2) Mouse mAb #5356.
Western blot analysis of extracts from various cell lines using HDAC6 (D2E5) Rabbit mAb #7558.
Western blot analysis of extracts from various cell lines using HDAC4 (D15C3) Rabbit mAb #7628.
The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.
Specificity / Sensitivity
The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform four mini-blot experiments.
Each antibody in the Histone Deacetylase (HDAC) Antibody Sampler Kit detects endogenous levels of its target.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human HDAC6, the amino terminus of human HDAC4 protein, synthetic peptides to the carboxy-terminal residues of human HDAC1 and HDAC2 proteins, or synthetic phosphopeptide corresponding to Ser155 of human HDAC7 protein. HDAC3 (7G6C5) monoclonal antibody is produced by immunizing animals with recombinant human HDAC3 protein.
Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).
- Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.
- Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202.
- Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53.
- Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16.
- Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83.
- Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100.
- Vigushin, D.M. and Coombes, R.C. (2004) Curr. Cancer Drug Targets 4, 205-218.
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