Product Pathways - MAPK Signaling
Phospho-MAPK Family Rabbit mAb Sampler Kit #9938
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|---|---|
| Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb # 4370 | 40 microliters | W IP IHC-P IF-IC F | H M R Mk Mi Hm B Dr Z | 44, 42 kDa | Rabbit |
| Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb # 4668 | 40 microliters | W IP IHC-P | H M R Dr | 46, 54 | Rabbit |
| Phospho-p38 MAPK (Thr180/Tyr182)(12F8) Rabbit mAb (IHC Preferred) # 4631 | 40 microliters | W IHC-P IF-IC | H M R Mk Mi Dr | 43 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 50 microliters | Goat | |||
| U0126 (MEK1/2 Inhibitor) # 9903 | 0.3 milligrams |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Mi=Mink
Hm=Hamster
B=Bovine
Dr=Drosophila
Z=Zebra Fish
Specificity / Sensitivity
Phospho-p44/42 MAPK, Phospho-SAPK/ JNK and Phospho-p38 MAPK Rabbit mAbs recognize only the phosphorylated form of p44/42 MAPK, SAPK/JNK and p38 MAPK, respectively. They do not cross-react with other MAPK family members.
Western Blotting
Western blot analysis of extracts from 293, NIH/3T3 and C6 cells, treated with λ phosphatase or TPA as indicated, using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370 (upper), or p44/42 MAP Kinase (137F5) Rabbit mAb #4695 (lower).
Western Blotting
Western blot analysis of extracts from Jurkat, NIH/3T3, and PC12 cells, untreated or treated as indicated, using Phospho-p38 MAP Kinase (Thr180/Tyr182) (12F8) Rabbit mAb (IHC Preferred) #4631.
Western Blotting
Western blot analysis of extracts from 293 cells, untreated or UV-treated, NIH/3T3 cells, untreated or UV-treated and C6 cells, untreated or anisomycin-treated, using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or PMA-treated (right), using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370.
IHC-P (paraffin)
Immunohistochemistry of paraffin-embedded lung carcinoma, showing nuclear localization, using Phospho-p38 MAP Kinase (Thr180/Tyr182) (12F8) Rabbit Monoclonal Antibody (IHC Preferred) #4631.
IHC-P (paraffin)
Immunohistochemistry of paraffin-embedded human glioblastoma, untreated (left) or CIP phosphatase-treated (right), using Phospho-p38 MAP Kinase (Thr180/Tyr182) (12F8) Rabbit Monoclonal Antibody (IHC Preferred) #4631.
IHC-P (paraffin)
Immunohistochemistry of paraffin-embedded breast carcinoma, using Phospho-p38 MAP Kinase (Thr180/Tyr182) (12F8) Rabbit Monoclonal Antibody (IHC Preferred) #4631 (left) or the same antibody pre-incubated with antigen phospho-peptide (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded 293T cells untreated (left) or UV-treated (right) using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb in the presence of control peptide (left) or Phospho-SAPK/JNK (Thr183/Tyr185) Blocking Peptide #1215 (right).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370.
Flow Cytometry
Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of C2C12 cells, U0126-treated (left, #9903 10 μM for 1 h) or TPA-treated (right, #9905 200 nM for 15 min), using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with synthetic phosphorylated peptides corresponding to residues surrounding Thr202/Tyr204 of human p44 MAPK (Erk1), Thr183/Tyr185 of human SAPK or Thr180/Tyr182 of human p38. For MEK1/2 Inhibitor (U0126) #9903 information, see page 3.
Background
p44/42 MAPK (Erk1 and Erk2), SAPK/JNK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation and control of cellular responses to cytokines and stress.p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK).SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase.
- Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139.
- Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218.
- Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444.
- Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
- Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.
Application References
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Companion Products
- 9101 Phospho-p44/42 MAPK (Thr202/Tyr204) Antibody
- 9102 p44/42 MAP Kinase Antibody
- 9106 Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) Mouse mAb
- 9212 p38 MAP Kinase Antibody
- 9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb
- 9251 Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
- 9252 SAPK/JNK Antibody
- 9211 Phospho-p38 MAPK (Thr180/Tyr182) Antibody
- 9255 Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
- 9900 PD98059 (MEK1 Inhibitor)
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
