Product Pathways - Jak/Stat Pathway
Stat Antibody Sampler Kit #9939
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|---|---|
| Stat1 Antibody # 9172 | 40 microliters | W IP | H M R Mk (B) | 84, 91 | Rabbit |
| Stat3 Antibody # 9132 | 40 microliters | W IP IHC-P | H M R (B) | 79, 86 | Rabbit |
| Stat5 Antibody # 9363 | 40 microliters | W IP | H M R | 90 | Rabbit |
| Stat6 Antibody # 9362 | 40 microliters | W IP | H M R (B) | 110 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 100 microliters | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Specificity / Sensitivity
Each Stat antibody in the kit recognizes only its target protein, independent of phosphorylation state.
Western Blotting
Western blot analysis of extractsfrom HeLa cells, untreated or IFN-alpha-treated (100 ng/ml for 5 min.), using Stat3 Antibody #9132 (left) and Phospho-Stat3 (Tyr705) Antibody #9131 (right).
Western Blotting
Western blot analysis of extracts from SK-MEL-28 cells, untreated or IFN-alpha-treated (100 ng/ml), using Phospho-Stat1 (Tyr701) Antibody #9171 (upper) or Stat1 Antibody #9172 (lower).
Western Blotting
Western blot analysis of extracts from various cell lines using Stat6 Antibody #9362.
Western Blotting
Western blot analysis of extracts from K562 and BaF3 cell lines using Stat5 Antibody #9363.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear and cytoplasmic localization, using Stat3 Antibody #9132.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues of human Stat1, mouse Stat3, human Stat5 and human Stat6. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).
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Application References
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