Product Pathways - Glucose Metabolism
AICAR #9944
| MW | Source | Purity |
|---|---|---|
Description
AICAR is supplied at 250 mM concentration. For a 0.5 mM working concentration, dilute 1:500. For a 2 mM working concentration, dilute 1:125. Treat cells with desired concentration for 5-60 minutes. Please note: Appearance of AICAR solution should be a clear to yellow/brown solution without precipitation. If precipitation is observed, warm tightly capped vial at 37C until AICAR goes into solution. Agitation of warmed solution by vortex may be necessary.
Directions for Use
AICAR is supplied at 250 mM in water. Thaw completely before use. For a 0.5 mM working concentration, dilute 1:500. For a 2 mM working concentration, dilute 1:125. Treat cells with desired concentration for 5-60 minutes.
Background
AICAR (5-Aminoimidazole-4-carboxyamide ribonucleoside) is an adenosine analog taken up by muscle and phosphorylated to form 5-aminoimidazole-4-carboxamide-1--D-ribofuranosyl-5'-monophosphate (ZMP), which stimulates AMPK activity and glucose transport in skeletal muscle (1). AICAR has been used in studies measuring glucose uptake, diabetes and insulin resistance, and energy regulation during exercise. AICAR acts by entering nucleoside pools and significantly increasing levels of adenosine during periods of ATP breakdown (2).
- Sakoda, H. et al. (2002) Am. J. Physiol. Endocrinol. Metab. 282, E1239-E1244.
- Mullane, K. et al. (1993) Trends Cardiovasc. Med. 3, 227-234.
- Jakobsen, S. N. et al. (2001) J. Biol. Chem. 276, 46912-46916.
- Jessen, N. et al. (2003) J. Appl. Physiol. 94, 1373-1379.
- Giri, S. et al. (2004) J. Neurosci. 24, 479-487.
Application References
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