Product Pathways - Jak/Stat Pathway
Jak Isoform Sampler Kit #9945
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Jak1 Antibody # 3332 | 40 microliters | W IP | H M (R) (B) | 130 | Rabbit |
| Jak2 (D2E12) XP™ Rabbit mAb # 3230 | 40 microliters | W IP IHC-P IF-IC F | H M R (Mk) | 125 | Rabbit IgG |
| Jak3 Antibody # 3775 | 40 microliters | W | H | 115 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 50 microliters | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Specificity / Sensitivity
Each Jak isoform antibody recognizes endogenous levels of the specified Jak isoform independent of its phosphorylation state. Neither antibody cross-reacts with the two other isoforms.
Western Blotting
Western blot analysis of extracts from K562, THP-1, TF-1 and BaF3 cell lines using Jak2 (D2E12) XP™ Rabbit mAb #3230.
Western Blotting
Western blot analysis of extracts from CTLL-2 and BaF3 cells using Jak1 Antibody #3332.
Western Blotting
Western blot analysis of recombinant proteins containing the kinase domain of Jak1, Jak2 and Jak3 (5 ng each), using Jak3 Antibody #3775.
Western Blotting
Western blot analysis of extracts from Jurkat cells using Jak3 Antibody #3775.
Description
The Jak Isoform Antibody Sampler Kit provides an economical means to examine several Jak isoforms. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.
Source / Purification
Each isoform-specific antibody is produced by immunizing animals with a synthetic peptide that is specific for the Jak isoform. Jak1 and Jak3 antibodies are purified by protein A and peptide affinity chromatography. Each Jak antibody included in this kit is specific and does not cross-react with the other isoforms.
Background
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
- Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-1635.
- VanderKuur, J. et al. (1995) J. Biol. Chem. 270, 7587-7593.
- Argetsinger, L.S. et al. (1995) J. Biol. Chem. 270, 14685-14692.
- Zhu, T. et al. (1998) J. Biol. Chem. 273, 10682-10689.
Application References
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Protocols
- 3230:
- Flow, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
- 3332:
- Immunoprecipitation, Western Blotting
- 3775:
- Western Blotting
Companion Products
- 3776 Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb
- 3771 Phospho-Jak2 (Tyr1007/1008) Antibody
- 3774 Phospho-Jak2 (Tyr221) Antibody
- 3331 Phospho-Jak1 (Tyr1022/1023) Antibody
- 9145 Phospho-Stat3 (Tyr705) (D3A7) XP™ Rabbit mAb
- 9131 Phospho-Stat3 (Tyr705) Antibody
- 9351 Phospho-Stat5 (Tyr694) Antibody
- 9132 Stat3 Antibody
- 9139 Stat3 (124H6) Mouse mAb
- 4904 Stat3 (79D7) Rabbit mAb
- 9363 Stat5 Antibody
- 9175 Stat1 (42H3) Rabbit mAb
- 9939 Stat Antibody Sampler Kit
- 1039 Jak2 Blocking Peptide
- 7751 Jak2 Kinase
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
