Product Pathways - Jak/Stat Pathway
Jak Isoform Sampler Kit #9945
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|---|---|
| Jak1 Antibody # 3332 | 40 microliters | W IP | H M (R) (B) | 130 | Rabbit |
| Jak2 (24B11) Rabbit mAb # 3229 | 40 microliters | W IP IHC-P | H M R B | 125 | Rabbit |
| Jak3 Antibody # 3775 | 40 microliters | W | H | 115 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 50 microliters | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Specificity / Sensitivity
Each Jak isoform antibody recognizes endogenous levels of the specified Jak isoform independent of its phosphorylation state. Neither antibody cross-reacts with the two other isoforms.
Western Blotting
Western blot analysis of extracts from LnCap, K562 and BaF3 cell lines, using Jak2 (24B11) Rabbit mAb.
Western Blotting
Western blot analysis of recombinant proteins containing the kinase domain of Jak1, Jak2 and Jak3 (5 ng each), using Jak3 Antibody.
Source / Purification
Each isoform-specific antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) that is specific for the Jak isoform. Jak1 and Jak3 antibodies are purified by protein A and peptide affinity chromatography. Each Jak antibody included in this kit is specific and does not cross-react with the other isoforms.
Background
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
- Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-1635.
- VanderKuur, J. et al. (1995) J. Biol. Chem. 270, 7587-7593.
- Argetsinger, L.S. et al. (1995) J. Biol. Chem. 270, 14685-14692.
- Zhu, T. et al. (1998) J. Biol. Chem. 273, 10682-10689.
Application References
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Companion Products
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- 9939 Stat Antibody Sampler Kit
- 9131 Phospho-Stat3 (Tyr705) Antibody
- 9132 Stat3 Antibody
- 7751 Jak2 Kinase
- 9145 Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb
- 9139 Stat3 (124H6) Mouse mAb
- 4904 Stat3 (79D7) Rabbit mAb
- 9175 Stat1 (42H3) Rabbit mAb (Human Specific)
- 1039 Jak2 Blocking Peptide