Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Jak Isoform Sampler Kit #9945

Kit Includes Quantity Applications Reactivity MW (kDa) Source
Jak1 Antibody # 3332 40 microliters W IP H M (R) (B) 130 Rabbit
Jak2 (24B11) Rabbit mAb # 3229 40 microliters W IP IHC-P H M R B 125 Rabbit
Jak3 Antibody # 3775 40 microliters W H 115 Rabbit
Anti-rabbit IgG, HRP-linked Antibody # 7074 50 microliters Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine

Specificity / Sensitivity

Each Jak isoform antibody recognizes endogenous levels of the specified Jak isoform independent of its phosphorylation state. Neither antibody cross-reacts with the two other isoforms.

Western Blotting

Western Blotting

Western blot analysis of extracts from LnCap, K562 and BaF3 cell lines, using Jak2 (24B11) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from CTLL-2 and BaF3 cells, using Jak1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of recombinant proteins containing the kinase domain of Jak1, Jak2 and Jak3 (5 ng each), using Jak3 Antibody.


Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, using Jak3 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Jak2 (24B11) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

Source / Purification

Each isoform-specific antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) that is specific for the Jak isoform. Jak1 and Jak3 antibodies are purified by protein A and peptide affinity chromatography. Each Jak antibody included in this kit is specific and does not cross-react with the other isoforms.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-1635.
  3. VanderKuur, J. et al. (1995) J. Biol. Chem. 270, 7587-7593.
  4. Argetsinger, L.S. et al. (1995) J. Biol. Chem. 270, 14685-14692.
  5. Zhu, T. et al. (1998) J. Biol. Chem. 273, 10682-10689.

Application References

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