Product Pathways - Akt Signaling
Wortmannin #9951
Directions for Use
For 1 mM stock, dissolve 1 mg in 2.32 ml DMSO. For experiments with cultured cells, CST recommends treating with wortmannin at concentrations between 0.2 µm and 1 µm, for one hour prior to, and for the duration of, the stimulation. See MSDS for further information.
Western Blotting
Western blot analysis of extracts from untreated, wortmannin and/or LY294002 treated Jurkat cells, using Phospho-Akt (Ser473) Antibody #9271 (A), Phospho-Akt (Thr308) Antibody #9275 (B), Akt Antibody #9272 (C) and Phospho-GSK-3alpha/beta (Ser21/9) Antibody #9331 (D).
Background
Wortmannin is a very potent, specific and direct inhibitor of PI3 kinase, originally derived from fungus (1,2). The inhibition is irreversible and noncompetitive. Wortmannin does not inhibit PI4 kinase, protein kinase C or protein tyrosine kinase (3).
- Nakanishi, S. et al. (1992) J. Biol. Chem. 267, 2157-2163.
- Arcaro, A. and Wymann, M.P. (1993) Biochem. J. 296, 297-301.
- Powis, G. et al. (1994) Cancer Res. 54, 5241-5248.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9271 Phospho-Akt (Ser473) Antibody
- 9275 Phospho-Akt (Thr308) Antibody
- 9272 Akt Antibody
- 9331 Phospho-GSK-3α/β (Ser21/9) Antibody
- 4056 Phospho-Akt (Thr308) (244F9) Rabbit mAb
- 4058 Phospho-Akt (Ser473) (193H12) Rabbit mAb
- 9940 Akt Isoform Sampler Kit
- 9280 PhosphoPlus® Akt Activation Kit
Precautions: This compound is only sold for use in extremely dilute solutions for biological research. No other use is intended and any other use involves substantial hazards. This compound should never be handled in powder or aerosol form or in any other form susceptible to uncontrolled release in the laboratory, even in very small quantities.
