Product Pathways - Apoptosis / Autophagy
Staurosporine #9953
| MW | Source | Purity |
|---|---|---|
| 466 g/Mol | Culture broth of Streptomyces staurospores. |
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or Staurosporine-treated (3 hours), showing PARP cleavage as evidence of induction of apoptosis, using Cleaved PARP Antibody #9541 (upper) or PARP Antibody #9542 (lower).
Directions for Use
Dissolve the lyophilized alkaloid in DMSO or Methanol. For a 1 mM solution, dissolve in 0.536 mL.
Background
Staurosporine is an alkaloid isolated from the culture broth of Streptomyces staurosporesa. It is a potent, cell permeable protein kinase C inhibitor with an IC50 of 0.7 nM. At higher concentration (1-20 nM), staurosporine also inhibits other kinases such as PKA, PKG, CAMKII and Myosin light chain kinase (MLCK) (1). At 50-100 nM, it is a functional neurotrophin agonist, promoting neurite outgrowth in neuroblastoma, pheochromocytoma and brain primary neuronal cultures. At 0.2- 1 |M, staurosporine induces cell apoptosis (2, 3).
- Ruegg, U.T. and Burgess, G.M. (1989) Trends Pharmacol. Sci. 10, 218-220.
- Couldwell , W. T. et al. (1994) FEBS Lett. 345, 43-46.
- Yue, T. L. et al. (1998) J. Mol. Cell. Cardiol. 30, 495-507.
Application References
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