Product Pathways - Jak/Stat Pathway
Interleukin-6 (IL-6) #9954
| MW | Source | Purity |
|---|---|---|
| 23.7 | Human Recombinant Protein |
Description
Human IL-6 cDNA was subcloned into a prokaryotic expression vector, expressed in E. coli, and recombinant human Interleukin-6 (rhIL-6) protein was purified.
Concentration and Specific Activity
100 µg/ml
Western Blotting
Western blot analysis of extracts from human myeloma cells stimulated with rhIL-6 (100 ng/ml for 10 min.), using Phospho-Stat1(Tyr701) Antibody #9171 (upper A), Stat1 Antibody #9172 (lower A), Phospho-Stat3(Tyr705) Antibody #9131 (upper B), Stat3 Antibody #9132 (lower B), Phospho-Jak1(Tyr1022/1023) Antibody #3331 (upper C) and Jak1 Antibody #3332 (lower C).
Directions for Use
CST recommends using 50-100 ng/ml for stimulation of IL-6 signaling.
Background
Interleukin-6 (IL-6) is a pleiotropic cytokine with central roles in immune regulation, inflammation, hematopoiesis, and oncogenesis. Its biological activities are shared by other IL-6-family members such as leukemia inhibitory factor and oncostatin M (1). To exert its biological effects, IL-6 must bind to the IL-6 receptor (IL-6R), composed of two alpha chains (IL-6Ralpha , 80 kDa) and two beta chains (IL-6Rbeta or gp130, 130 kDa). Two moieties of IL-6 and two pairs of these receptor chains form a functional hexameric IL-6R complex. The subsequent intracellular signaling events are activated via gp130, which is the common signal transducer of cytokines related to IL-6. Activation of the IL-6R stimulates at least three major signaling pathways : JAK/STAT, PI-3 kinase and MAPK signaling pathways (2,3).
- Ito, H. (2003) Curr. Drug Targets Inflamm. Allergy 2, 125-130.
- Taniguchi, T. (1995) Science 268, 251-255.
- Boulton, T. G. et al. (1994) J. Biol. Chem. 269, 11648-11655.
Application References
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Companion Products
- 9170 PhosphoPlus® Stat1 (Tyr701) Antibody Kit
- 9171 Phospho-Stat1 (Tyr701) Antibody
- 9172 Stat1 Antibody
- 9130 PhosphoPlus® Stat3 (Tyr705) Antibody Kit
- 9131 Phospho-Stat3 (Tyr705) Antibody
- 9132 Stat3 Antibody
- 9138 Phospho-Stat3 (Tyr705) (3E2) Mouse mAb
- 9139 Stat3 (124H6) Mouse mAb
- 3331 Phospho-Jak1 (Tyr1022/1023) Antibody
- 3332 Jak1 Antibody
- 9803 Cell Lysis Buffer (10X)
- 9806 RIPA Buffer (10X)
- 7722 Blue Loading Buffer Pack
- 7723 Red Loading Buffer Pack
