Product Pathways - NF-kB Signaling
Phospho-IKKα/β (Ser176/180) Antibody Sampler Kit #9958
|9958S||1 Kit (3 x 4 western blots)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb #2078||40 µl||W||H, M||R, Mk, B||85 (IKKalpha), 87 (IKKbeta)||Rabbit|
|Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697||40 µl||W, IHC-P, IHC-F||H, M, R, Mk||B||85 IKK-alpha 87 IKK-beta||Rabbit IgG|
|Phospho-IKKα/β (Ser176/180) Antibody II #2694||40 µl||W||H, M, R, Mk||85 IKK-alpha 87 IKK-beta||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Western blot analysis of extracts from differentiated THP-1 cells, untreated or LPS-treated for 15 minutes, using Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb #2078 (upper), IKKα Antibody #2682 (middle) and IKKβ (2C8) Rabbit mAb #2370 (lower).
Western blot analysis of extracts from HeLa cells, treated with TNF-α (20ng/ml) for the indicated times, using Phospho-IKKα/β (Ser176/180) Antibody II #2694.
Western blot analysis of extracts from HeLa and NIH/3T3 cells, treated with TNF-α (20 ng/ml) and calyculin A #9902 (50 nM), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697.
The Phospho-IKKalpha/beta (Ser176/180) Antibody Sampler Kit contains reagents to examine protein levels of IKKalpha when phosphorylated at Ser176/180 and IKKbeta when phosphorylated at Ser177/181. The kit contains primary and secondary antibodies to perform four Western blots with each antibody.
Specificity / Sensitivity
Phospho-IKKα/β (Ser176/180) Antibody, Phospho-IKKα/β (Ser176/180) Antibody II, and Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb detect IKKα only when phosphorylated at Ser176/180 and IKKβ only when phosphorylated at Ser177/181.
Source / Purification
Polyclonal antibodies #2687 and #2694 are produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα and Ser177/181 of IKKβ, respectively, and are purified by protein A and peptide affinity chromatography. Monoclonal antibody #2697 is produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα.
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.