Product Pathways - Adhesion
Cadherin-Catenin Antibody Sampler Kit #9961
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|---|---|
| β-Catenin Antibody (Carboxy-terminal Antigen) # 9587 | 40 microliters | W IP IHC-P IHC-F IF-F F | H M Mk Mi Hm B | 92 | Rabbit |
| α-E-Catenin Antibody # 3236 | 40 microliters | W IF-IC | H M R | 100 | Rabbit |
| N-Cadherin Antibody # 4061 | 40 microliters | W IP | H M R Mk | 140 | Rabbit |
| E-Cadherin Antibody # 4065 | 40 microliters | W IP IHC-P IF-IC | H M (B) | 135 | Rabbit |
| P-Cadherin Antibody # 2130 | 40 microliters | W IP IF-IC F | H | 120 | Rabbit |
| Pan-Cadherin Antibody # 4068 | 40 microliters | W IP IHC-P | H M R Mk B | 130 to 150 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 100 microliters | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Mi=Mink
Hm=Hamster
B=Bovine
Specificity / Sensitivity
Each antibody in the Cadherin-Catenin Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Pan-Cadherin Antibody #4068 detects endogenous levels of total E-, P-, N-, R-, VE- and K-cadherin proteins.
Western Blotting
Western blot analysis of extracts from various cell lines using α-E-Catenin Antibody #3236.
Western Blotting
Western blot analysis of extracts from various cell lines using N-Cadherin Antibody #4061.
Western Blotting
Western blot analysis of extracts from A431, MCF-7 and C2C12 cells using E-Cadherin Antibody #4065. Note the absence of signal from C2C12 cells, which express N-cadherin instead of E-cadherin.
Western Blotting
Western blot analysis of extracts from various cell lines using Pan-Cadherin Antibody #4068.
Western Blotting
Western blot analysis of total cell extracts from 293 and NIH/3T3 cells using β-Catenin Antibody (Carboxy-terminal Antigen) #9587.
IP
Immunoprecipitation of P-cadherin from A431 cell extracts using P-Cadherin Antibody #2130. Western blot analysis was performed with the same antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human bronchioalveolar cell carcinoma using Pan-Cadherin Antibody #4068 and showing membrane localization.
Flow Cytometry
Flow cytometric analysis of A431 cells using P-Cadherin Antibody #2130 (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent images of MCF-7 cells using E-Cadherin Antibody #4065 (green, left) compared to an isotype control (right). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
IF-IC
Confocal immunofluorescence image of mouse colon using β-Catenin Antibody (Carboxy-terminal Antigen) #9587 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Source / Purification
The β-catenin Antibody #9587 was produced by immunizing rabbits with a synthetic peptide (Blue Carrier-coupled) corresponding to the carboxy-terminus of human β-catenin. α-E-Catenin Antibody #3236 was produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of human α-E-catenin. The total cadherin antibodies #4061, #2130 and #4065 were produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues within human N-cadherin, human P-cadherin and human E-cadherin, respectively. Pan-cadherin Antibody #4068 was produced by immunizing rabbits with two synthetic peptides (KLH-coupled) corresponding to two conserved regions of human N-cadherin.
Background
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of cadherins that are transmembrane proteins that bind cadherins on adjacent cells in a calcium dependent manner. On the cytoplasmic side of adherens junctions, the cadherins associate with β-catenin, γ-catenin and p120 catenin (δ). β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Recent studies indicate that cancer cells exhibit increased N-cadherin and diminished E-cadherin expression. E-cadherin is considered a suppressor of invasive cancer cell growth and this change in cadherin expression associated with cancer progression is termed the ?cadherin switch?. β-catenin is one of the key downstream effectors in the Wnt signaling pathway and has been implicated in early embryonic development and tumorigenesis (3-5).
- Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235.
- Christofori, G. (2003) EMBO J. 22, 2318-2323.
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9581 β-Catenin Antibody (Amino-terminal Antigen)
- 9587 β-Catenin Antibody (Carboxy-terminal Antigen)
- 9562 β-Catenin Antibody
- 3236 α-E-Catenin Antibody
- 3240 α-E-Catenin (23B2) Rabbit mAb
- 4065 E-Cadherin Antibody
- 4061 N-Cadherin Antibody
- 2130 P-Cadherin Antibody
- 4068 Pan-Cadherin Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
