Product Pathways - Adhesion
Cadherin-Catenin Antibody Sampler Kit #9961
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| α-E-Catenin (23B2) Rabbit mAb #3240 | 40 µl | W IP | H M Mk | 100 | Rabbit IgG |
| N-Cadherin Antibody #4061 | 40 µl | W | H M R Mk (Dg) | 140 | Rabbit |
| E-Cadherin (24E10) Rabbit mAb #3195 | 40 µl | W IHC-P IHC-F IF-IC F | H M (B) (Dg) (Pg) | 135 | Rabbit IgG |
| P-Cadherin (C13F9) Rabbit mAb #2189 | 40 µl | W IF-IC | H (Mk) | 120 | Rabbit IgG |
| Pan-Cadherin (28E12) Rabbit mAb #4073 | 40 µl | W | H M R | 130-150 | Rabbit IgG |
| β-Catenin (D10A8) XP® Rabbit mAb #8480 | 40 µl | W IP IHC-P IHC-F IF-F IF-IC F ChIP | H M R Mk (Z) (B) (Pg) (GP) (Hr) | 92 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
ChIP=Chromatin IP
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Z=Zebrafish
B=Bovine
Dg=Dog
Pg=Pig
GP=Guinea Pig
Hr=Horse
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the Cadherin-Catenin Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Pan-Cadherin (28E12) Rabbit mAb #4073 detects endogenous levels of total cadherin proteins.
Western Blotting
Western blot analysis of extracts from various cell lines using P-Cadherin (C13F9) Rabbit mAb #2189.
Western Blotting
Western blot analysis of extracts from various cell lines using E-Cadherin (24E10) Rabbit mAb #3195.
Western Blotting
Western blot analysis of extracts from HUVEC, NIH/3T3, MCF-7, ZR-75 and A431 cells using α-E-Catenin (23B2) Rabbit mAb #3240.
Western Blotting
Western blot analysis of extracts from various cell lines using N-Cadherin Antibody #4061.
Western Blotting
Western blot analysis of extracts from various cell lines using Pan-Cadherin (28E12) Rabbit mAb #4073.
Western Blotting
Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP® Rabbit mAb #8480.
Description
This Cadherin-Catenin Antibody Sampler kit contains reagents to examine the total protein levels of key proteins found in cell-cell adherens junctions. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.
Source / Purification
Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the residues within the amino-terminus of human N-cadherin and is purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human α-E-catenin, residues near the carboxy terminus of human P-cadherin, residues surrounding 780 of human E-cadherin, residues surrounding Pro714 of human ß-catenin protein, and a synthetic peptide corresponding to a conserved region of human N-, R-, E- and P-Cadherin.
Background
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of cadherins that are transmembrane proteins that bind cadherins on adjacent cells in a calcium dependent manner. On the cytoplasmic side of adherens junctions, the cadherins associate with β-catenin, γ-catenin and p120 catenin (δ). β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Recent studies indicate that cancer cells exhibit increased N-cadherin and diminished E-cadherin expression. E-cadherin is considered a suppressor of invasive cancer cell growth and this change in cadherin expression associated with cancer progression is termed the “cadherin switch”. β-catenin is one of the key downstream effectors in the Wnt signaling pathway and has been implicated in early embryonic development and tumorigenesis (3-5).
- Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235.
- Christofori, G. (2003) EMBO J. 22, 2318-2323.
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Protocols
- 2189:
- Immunofluorescence, Western Blotting
- 3195:
- Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Western Blotting
- 3240:
- Immunoprecipitation, Western Blotting
- 4061:
- Western Blotting
- 4073:
- Western Blotting
- 8480:
- ChIP Agarose, ChIP Magnetic, Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
Companion Products
- 9581 β-Catenin Antibody (Amino-terminal Antigen)
- 9582 β-Catenin (6B3) Rabbit mAb
- 9562 β-Catenin Antibody
- 3236 α-E-Catenin Antibody
- 3240 α-E-Catenin (23B2) Rabbit mAb
- 4061 N-Cadherin Antibody
- 2130 P-Cadherin Antibody
- 4068 Pan-Cadherin Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
For Research Use Only. Not For Use In Diagnostic Procedures.
