Product Pathways - Adhesion

Cadherin-Catenin Antibody Sampler Kit #9961

Kit Includes	Quantity	Applications	Reactivity	MW (kDa)	Source
β-Catenin Antibody (Carboxy-terminal Antigen) # 9587	40 microliters	W IP IHC-P IHC-F IF-F F ChIP	H M Mk Mi Hm B (Pg) (C) (X) (Dg)	92	Rabbit
α-E-Catenin Antibody # 3236	40 microliters	W IF-IC	H M R	100	Rabbit
N-Cadherin Antibody # 4061	40 microliters	W IP	H M R Mk	140	Rabbit
E-Cadherin Antibody # 4065	40 microliters	W IP IHC-P IF-IC	H M (B) (Dg)	135	Rabbit
P-Cadherin Antibody # 2130	40 microliters	W IP IF-IC F	H	120	Rabbit
Pan-Cadherin Antibody # 4068	40 microliters	W IP IHC-P	H M R Mk B Z	130 to 150	Rabbit
Anti-rabbit IgG, HRP-linked Antibody # 7074	100 microliters				Goat

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-F=Immunofluorescence (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Mi=Mink Pg=Pig C=Chicken Hm=Hamster B=Bovine X=Xenopus Z=Zebrafish Dg=Dog

Specificity / Sensitivity

Each antibody in the Cadherin-Catenin Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Pan-Cadherin Antibody #4068 detects endogenous levels of total E-, P-, N-, R-, VE- and K-cadherin proteins.

Western Blotting

Western blot analysis of extracts from various cell lines using α-E-Catenin Antibody #3236.

Western Blotting

Western blot analysis of extracts from various cell lines using N-Cadherin Antibody #4061.

Western Blotting

Western blot analysis of extracts from A431, MCF-7 and C2C12 cells using E-Cadherin Antibody #4065. Note the absence of signal from C2C12 cells, which express N-cadherin instead of E-cadherin.

Western Blotting

Western blot analysis of extracts from various cell lines using Pan-Cadherin Antibody #4068.

Western Blotting

Western blot analysis of total cell extracts from 293 and NIH/3T3 cells using β-Catenin Antibody (Carboxy-terminal Antigen) #9587.

IP

Immunoprecipitation of P-cadherin from A431 cell extracts using P-Cadherin Antibody #2130. Western blot analysis was performed with the same antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human bronchioalveolar cell carcinoma using Pan-Cadherin Antibody #4068 and showing membrane localization.

Flow Cytometry

Flow cytometric analysis of A431 cells using P-Cadherin Antibody #2130 (blue) compared to a nonspecific negative control antibody (red).

IF-IC

Confocal immunofluorescent images of MCF-7 cells using E-Cadherin Antibody #4065 (green, left) compared to an isotype control (right). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

IF-IC

Confocal immunofluorescence image of mouse colon using β-Catenin Antibody (Carboxy-terminal Antigen) #9587 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Source / Purification

The β-catenin Antibody #9587 was produced by immunizing rabbits with a synthetic peptide (Blue Carrier-coupled) corresponding to the carboxy-terminus of human β-catenin. α-E-Catenin Antibody #3236 was produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of human α-E-catenin. The total cadherin antibodies #4061, #2130 and #4065 were produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues within human N-cadherin, human P-cadherin and human E-cadherin, respectively. Pan-cadherin Antibody #4068 was produced by immunizing rabbits with two synthetic peptides (KLH-coupled) corresponding to two conserved regions of human N-cadherin.

Background

Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of cadherins that are transmembrane proteins that bind cadherins on adjacent cells in a calcium dependent manner. On the cytoplasmic side of adherens junctions, the cadherins associate with β-catenin, γ-catenin and p120 catenin (δ). β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Recent studies indicate that cancer cells exhibit increased N-cadherin and diminished E-cadherin expression. E-cadherin is considered a suppressor of invasive cancer cell growth and this change in cadherin expression associated with cancer progression is termed the ?cadherin switch?. β-catenin is one of the key downstream effectors in the Wnt signaling pathway and has been implicated in early embryonic development and tumorigenesis (3-5).

Application References

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Protocols

2130:: Flow, Immunofluorescence, Immunoprecipitation, Western Blotting
3236:: Immunofluorescence, Western Blotting
4061:: Immunoprecipitation, Western Blotting
4065:: IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting
4068:: IHC / Paraffin, Immunoprecipitation, Western Blotting
9587:: Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting

Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.