Product Pathways - Adhesion
Cadherin-Catenin Antibody Sampler Kit #9961
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| β-Catenin (6B3) Rabbit mAb #9582 | 40 µl | W IP IHC-P IHC-F | H M R Mk (X) (B) (Dg) (Pg) (Hr) | 92 | Rabbit IgG |
| α-E-Catenin (23B2) Rabbit mAb #3240 | 40 µl | W IP | H M Mk | 100 | Rabbit IgG |
| N-Cadherin Antibody #4061 | 40 µl | W | H M R Mk | 140 | Rabbit |
| E-Cadherin (24E10) Rabbit mAb #3195 | 40 µl | W IHC-P IHC-F IF-IC F | H M (Dg) (Pg) | 135 | Rabbit IgG |
| P-Cadherin (C13F9) Rabbit mAb #2189 | 40 µl | W IF-IC | H (Mk) | 120 | Rabbit IgG |
| Pan-Cadherin (28E12) Rabbit mAb #4073 | 40 µl | W | H M R | 130-150 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
X=Xenopus
B=Bovine
Dg=Dog
Pg=Pig
Hr=Horse
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the Cadherin-Catenin Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Pan-Cadherin (28E12) Rabbit mAb #4073 detects endogenous levels of total cadherin proteins.
Western Blotting
Western blot analysis of extracts from various cell lines using P-Cadherin (C13F9) Rabbit mAb #2189.
Western Blotting
Western blot analysis of extracts from various cell lines using E-Cadherin (24E10) Rabbit mAb #3195.
Western Blotting
Western blot analysis of extracts from HUVEC, NIH/3T3, MCF-7, ZR-75 and A431 cells using α-E-Catenin (23B2) Rabbit mAb #3240.
Western Blotting
Western blot analysis of extracts from various cell lines using N-Cadherin Antibody #4061.
Western Blotting
Western blot analysis of extracts from various cell lines using Pan-Cadherin (28E12) Rabbit mAb #4073.
Western Blotting
Western blot analysis of total cell lysates from 293, Rat2 and COS cells using β-Catenin (6B3) Rabbit mAb #9582.
Description
This Cadherin-Catenin Antibody Sampler kit contains reagents to examine the total protein levels of key proteins found in cell-cell adherens junctions. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human β-catenin and residues within human N-cadherin and are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human α-E-catenin, residues near the carboxy terminus of human P-cadherin, residues surrounding 780 of human E-cadherin, and a synthetic peptide corresponding to a conserved region of human N-, R-, E- and P-Cadherin.
Background
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of cadherins that are transmembrane proteins that bind cadherins on adjacent cells in a calcium dependent manner. On the cytoplasmic side of adherens junctions, the cadherins associate with β-catenin, γ-catenin and p120 catenin (δ). β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Recent studies indicate that cancer cells exhibit increased N-cadherin and diminished E-cadherin expression. E-cadherin is considered a suppressor of invasive cancer cell growth and this change in cadherin expression associated with cancer progression is termed the “cadherin switch”. β-catenin is one of the key downstream effectors in the Wnt signaling pathway and has been implicated in early embryonic development and tumorigenesis (3-5).
- Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235.
- Christofori, G. (2003) EMBO J. 22, 2318-2323.
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Protocols
- 2189:
- Immunofluorescence, Western Blotting
- 3195:
- Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Western Blotting
- 3240:
- Immunoprecipitation, Western Blotting
- 4061:
- Western Blotting
- 4073:
- Western Blotting
- 9582:
- IHC / Frozen, IHC / Paraffin, Immunoprecipitation, Western Blotting
Companion Products
- 9581 β-Catenin Antibody (Amino-terminal Antigen)
- 9582 β-Catenin (6B3) Rabbit mAb
- 9562 β-Catenin Antibody
- 3236 α-E-Catenin Antibody
- 3240 α-E-Catenin (23B2) Rabbit mAb
- 4061 N-Cadherin Antibody
- 2130 P-Cadherin Antibody
- 4068 Pan-Cadherin Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
