Cell Signaling Technology

Product Pathways - Adhesion

Cadherin-Catenin Antibody Sampler Kit #9961

Kit Includes Quantity Applications Reactivity MW (kDa) Source
β-Catenin Antibody (Carboxy-terminal Antigen) # 9587 40 microliters W IP IHC-P IHC-F IF-F F H M Mk Mi Hm B 92 Rabbit
α-E-Catenin Antibody # 3236 40 microliters W IF-IC H M R 100 Rabbit
N-Cadherin Antibody # 4061 40 microliters W IP H M R Mk 140 Rabbit
E-Cadherin Antibody # 4065 40 microliters W IP IHC-P IF-IC H M (B) 135 Rabbit
P-Cadherin Antibody # 2130 40 microliters W IP IF-IC F H 120 Rabbit
Pan-Cadherin Antibody # 4068 40 microliters W IP IHC-P H M R Mk B 130 to 150 Rabbit
Anti-rabbit IgG, HRP-linked Antibody # 7074 100 microliters Goat

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-F=Immunofluorescence (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Mi=Mink  Hm=Hamster  B=Bovine

Specificity / Sensitivity

Each antibody in the Cadherin-Catenin Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Pan-Cadherin Antibody #4068 detects endogenous levels of total E-, P-, N-, R-, VE- and K-cadherin proteins.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using α-E-Catenin Antibody #3236.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using N-Cadherin Antibody #4061.

Western Blotting

Western Blotting

Western blot analysis of extracts from A431, MCF-7 and C2C12 cells using E-Cadherin Antibody #4065. Note the absence of signal from C2C12 cells, which express N-cadherin instead of E-cadherin.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Pan-Cadherin Antibody #4068.

Western Blotting

Western Blotting

Western blot analysis of total cell extracts from 293 and NIH/3T3 cells using β-Catenin Antibody (Carboxy-terminal Antigen) #9587.

IP

IP

Immunoprecipitation of P-cadherin from A431 cell extracts using P-Cadherin Antibody #2130. Western blot analysis was performed with the same antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human bronchioalveolar cell carcinoma using Pan-Cadherin Antibody #4068 and showing membrane localization.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of A431 cells using P-Cadherin Antibody #2130 (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent images of MCF-7 cells using E-Cadherin Antibody #4065 (green, left) compared to an isotype control (right). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


IF-IC

IF-IC

Confocal immunofluorescence image of mouse colon using β-Catenin Antibody (Carboxy-terminal Antigen) #9587 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Source / Purification

The β-catenin Antibody #9587 was produced by immunizing rabbits with a synthetic peptide (Blue Carrier-coupled) corresponding to the carboxy-terminus of human β-catenin. α-E-Catenin Antibody #3236 was produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of human α-E-catenin. The total cadherin antibodies #4061, #2130 and #4065 were produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues within human N-cadherin, human P-cadherin and human E-cadherin, respectively. Pan-cadherin Antibody #4068 was produced by immunizing rabbits with two synthetic peptides (KLH-coupled) corresponding to two conserved regions of human N-cadherin.

Background

Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of cadherins that are transmembrane proteins that bind cadherins on adjacent cells in a calcium dependent manner. On the cytoplasmic side of adherens junctions, the cadherins associate with β-catenin, γ-catenin and p120 catenin (δ). β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Recent studies indicate that cancer cells exhibit increased N-cadherin and diminished E-cadherin expression. E-cadherin is considered a suppressor of invasive cancer cell growth and this change in cadherin expression associated with cancer progression is termed the ?cadherin switch?. β-catenin is one of the key downstream effectors in the Wnt signaling pathway and has been implicated in early embryonic development and tumorigenesis (3-5).

  1. Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235.
  2. Christofori, G. (2003) EMBO J. 22, 2318-2323.
  3. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
  4. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59-88.
  5. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.

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