Product Pathways - Protein Translation
mTOR Pathway Antibody Sampler Kit #9964
|9964S||1 Kit (6 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Phospho-mTOR (Ser2481) Antibody #2974||40 µl||W||H, M, R, Mk||289||Rabbit|
|mTOR (7C10) Rabbit mAb #2983||40 µl||W, IHC-P, IF-IC, F||H, M, R, Mk||Hr||289||Rabbit|
|Raptor (24C12) Rabbit mAb #2280||40 µl||W, IP||H, M, R, Mk||150||Rabbit IgG|
|Rictor (53A2) Rabbit mAb #2114||40 µl||W||H, M, R, Mk||200||Rabbit IgG|
|GβL (86B8) Rabbit mAb #3274||40 µl||W, IP||H, M, R, Mk||37||Rabbit IgG|
|Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb #5536||40 µl||W, IP, IF-IC||H, M, Mk||R, C, Pg, Hr||289||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Western blot analysis of extracts from various cell types using Rictor (53A2) Rabbit mAb #2114.
Western blot analysis of extracts from various cell types using Raptor (24C12) Rabbit mAb #2280.
Western blot analysis of extracts from 293 cells (starved for 30 hours), untreated or 20% FBS-treated for 30 minutes, using Phospho-mTOR (Ser2481) Antibody #2974.
Western blot analysis of extracts from various cell types using mTOR (7C10) Rabbit mAb #2983.
The mTOR Pathway Antibody Sampler Kit contains reagents to investigate the control of protein translation, cell growth, and proliferation through mTOR signaling within cells. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.
Specificity / Sensitivity
Each total antibody in the mTOR Pathway Sampler Kit recognizes only its specific target. Each phospho-specific antibody detects the intended target only when phosphorylated at the indicated site.
Source / Purification
Polyclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser2481 of human mTOR. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2448 of human mTOR, Gln1681 of human Rictor, Gln210 of human GβL and human Raptor.
The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).
The regulatory associated protein of mTOR (Raptor) interacts with mTOR to mediate mTOR signaling to downstream targets (10,11). Raptor binds to mTOR substrates, such as 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated substrate phosphorylation (12,13). Binding of the FKBP12-rapamycin complex to mTOR inhibits mTOR-raptor interaction, which suggests a mechanism for the inhibition of mTOR signaling by rapamycin (14). This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL (15). GβL is part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR) and may mediate rictor-mTOR signaling to PKCα and other downstream targets (16). The rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB at Ser473, which is required for PDK1 phosphorylation of Akt/PKB at Thr308 and full activation of Akt/PKB (17).
- Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
- Brown, E.J. et al. (1994) Nature 369, 756-8.
- Sabatini, D.M. et al. (1994) Cell 78, 35-43.
- Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
- Dennis, P.B. et al. (2001) Science 294, 1102-5.
- Fang, Y. et al. (2001) Science 294, 1942-5.
- Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
- Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
- Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.
- Hara, K. et al. (2002) Cell 110, 177-189.
- Kim, D.H. et al. (2002) Cell 110, 163-175.
- Beugnet, A. et al. (2003) J. Biol. Chem. 278, 40717-40722.
- Nojima, H. et al. (2003) J. Biol. Chem. 278, 15461-15464.
- Oshiro, N. et al. (2004) Genes Cells 9, 359-366.
- Kim, D.H. et al. (2003) Mol. Cell 11, 895-904.
- Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.
- Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
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- 9862 mTOR Substrates Antibody Sampler Kit
- 9864 mTOR Regulation Antibody Sampler Kit
- 2971 Phospho-mTOR (Ser2448) Antibody
- 2974 Phospho-mTOR (Ser2481) Antibody
- 2983 mTOR (7C10) Rabbit mAb
- 2114 Rictor (53A2) Rabbit mAb
- 2280 Raptor (24C12) Rabbit mAb
- 3274 GβL (86B8) Rabbit mAb
- 2083 Phospho-Raptor (Ser792) Antibody
- 2691 PRAS40 (D23C7) XP® Rabbit mAb
- 2997 Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb
- 3360 RagC Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7075 Anti-biotin, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.