Cell Signaling Technology

Product Pathways - Translational Control

NCBP1/CBP80 Antibody #9983

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 80 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NCBP1/CBP80 recognizes endogenous levels of total NCBP1/CBP80 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val452 of human NCBP1/CBP80 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NCBP1/CBP80 Antibody.

Background

Nuclear cap-binding protein subunit 1 (NCBP1), also known as cap-binding protein 80 (CBP80), plays a role in nuclear pre-mRNA splicing (1,2). It has also been shown to function in the nonsense-mediated decay (NMD) of mRNAs where translation is prematurely terminated (3). NCBP1/CBP80 increases the efficiency of NMD by promoting the interaction of two active NMD components Upf1 and Upf2 (4).

  1. Izaurralde, E. et al. (1994) Cell 78, 657-68.
  2. Izaurralde, E. et al. (1995) Nature 376, 709-12.
  3. Ishigaki, Y. et al. (2001) Cell 106, 607-17.
  4. Hosoda, N. et al. (2005) Nat Struct Mol Biol 12, 893-901.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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