Product Pathways - Ca / cAMP / Lipid Signaling
Ionomycin, Calcium Salt #9995
| MW | Source | Purity |
|---|---|---|
| 747.1 |
Ionomycin structure
Western blot analysis of extracts from BAEC cells, untreated or treated with the indicated concentrations of ionomycin for 1 to 2 minutes. The effect of ionomycin treatment on eNOS phosphorylation at Thr495 was detected using Phospho-eNOS (Thr495) Antibody #9574 (upper). eNOS Antibody #9572 was used as a loading control (lower). Ionomycin-induced Ca2+ influx in BAEC cells results in rapid dephosphorylation of eNOS (Thr495).
Directions for Use
Ionomycin, calcium salt is supplied as 2.5 mg powder. Store at or below -20°C. Before use, dissolve powder in 1.1 ml DMSO to make a 3 mM ionomycin stock solution. For working concentrations of 1 μM-3 μM, dilute DMSO stock 1:3000 to 1:1000. Treat cells with the desired concentration for intended time. Ionomycin is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20°C.
Background
Ionomycin is a potent and selective calcium ionophore agent (1,2). The molecule acts as a motile Ca2+ carrier and enhances Ca2+ influx by direct stimulation of store-regulated cation entry across biological membranes (3). At the micromolar level, ionomycin can activate Ca2+/Calmodulin dependent kinase and phosphatase to stimulate gene expression (4). In human T cells, ionomycin induces hydrolysis of phosphoinositides and activates PKC to mediate T cell activation (5). Ionomycin treatment of human B cells induces the activation of calcium-dependent endonuclease and results in apoptosis (6). Ionomycin treatment of bovine aortic endothelial cells (BAECs) induces rapid dephosphorylation of eNOS at Thr495 and mediates eNOS activation (7).
- Liu, C. and Hermann, T.E. (1978) J Biol Chem 253, 5892-4.
- Kauffman, R.F. et al. (1980) J Biol Chem 255, 2735-9.
- Morgan, A.J. and Jacob, R. (1994) Biochem J 300 ( Pt 3), 665-72.
- Lobo, F.M. et al. (1999) J Immunol 162, 2057-63.
- Chatila, T. et al. (1989) J Immunol 143, 1283-9.
- Aagaard-Tillery, K.M. and Jelinek, D.F. (1995) J Immunol 155, 3297-307.
- Lin, M.I. et al. (2003) J Biol Chem 278, 44719-26.
Application References
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