Product Pathways - Ca / cAMP / Lipid Signaling
Ionomycin, Calcium Salt #9995
|9995S||2.5 mg||---||In Stock||---|
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- Molecular Formula:
- C41H70O9 • Ca2+
- Molecular Weight:
- 747.1 g/mol
Directions For Use
Ionomycin, calcium salt is supplied as 2.5 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1.1 ml DMSO to make a 3 mM ionomycin stock solution. For working concentrations of 1 μM-3 μM, dilute DMSO stock 1:3000 to 1:1000. Treat cells with the desired concentration for intended time. Ionomycin is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20ºC.
Western blot analysis of extracts from BAEC cells, untreated or treated with the indicated concentrations of ionomycin for 1 to 2 minutes. The effect of ionomycin treatment on eNOS phosphorylation at Thr495 was detected using Phospho-eNOS (Thr495) Antibody #9574 (upper). eNOS Antibody #9572 was used as a loading control (lower). Ionomycin-induced Ca2+ influx in BAEC cells results in rapid dephosphorylation of eNOS (Thr495).
Ionomycin is a potent and selective calcium ionophore agent (1,2). The molecule acts as a motile Ca2+ carrier and enhances Ca2+ influx by direct stimulation of store-regulated cation entry across biological membranes (3). At the micromolar level, ionomycin can activate Ca2+/Calmodulin dependent kinase and phosphatase to stimulate gene expression (4). In human T cells, ionomycin induces hydrolysis of phosphoinositides and activates PKC to mediate T cell activation (5). Ionomycin treatment of human B cells induces the activation of calcium-dependent endonuclease and results in apoptosis (6). Ionomycin treatment of bovine aortic endothelial cells (BAECs) induces rapid dephosphorylation of eNOS at Thr495 and mediates eNOS activation (7).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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