Cell Signaling Technology

Product Pathways - Metabolism

Oligomycin #9996

Molecular Formula:
C45H74O11 (for Oligomycin A)
Molecular Weight:
789.3 g/mol
Purity:
>99%

Directions for Use

Oligomycin is soluble in DMSO, acetone or ethanol.

Western Blotting

Western Blotting

Western blot analysis demonstrating optimal cell culture conditions for oligomycin treatment. 293 cells were cultured in the presence or absence of serum (O/N) and treated with or without Oligomycin (0.5µM, 30 min). Western blot analysis performed using Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb #4188.

Structure

Structure

Description

Oligomycin is supplied in lyophilized form.

Background

During oxidative phosphorylation in mitochondria, the electron transport system pumps H+ from the matrix into the intermembrane compartment. This process generates a H+ gradient across the mitochondrial inner membrane. This H+ gradient is used to drive mitochondrial FoF1 ATPase to convert ADP to ATP. Oligomycin, an antibiotic from Streptomyces diastatochromogenes, blocks oxidative phosphorylation by binding to the Fo part of the ATPase to inhibit its activity (1-3).

  1. Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2461-6.
  2. Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2467-74.
  3. Fessenden, J.M. and Racker, E. (1966) J Biol Chem 241, 2483-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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