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Western blot analysis of extracts from serum-starved 293 cells, untreated (lane 1), stimulated with TPA treatment (lanes 2 & 3), and treated with CIP and λ phosphatase (lane 3), using Phospho-PKC (pan) (βII Ser660) Antibody #9371 or PKCα Antibody #2056.

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Western blot analysis of extracts from serum-starved 293 cells, untreated (lane 1), stimulated with TPA treatment (lanes 2 & 3), and treated with CIP and λ phosphatase (lane 3), using Phospho-PKD/PKCμ (Ser916) Antibody #2051 or PKD/PKCμ Antibody #2052.

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Product Usage Information

TPA is supplied as a 1 mg powder. Store at -20C. Before use, dissolve powder in 1 ml DMSO to yield a 1.6 mM stock solution. For working concentration of 200 μM, add 125 μl stock solution to 875 μl DMSO. Treat cells with 200 nM TPA for 20-30 minutes.

TPA is soluble in DMSO and ethanol.


Storage: Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

616.84 g/mol


Purity:

>99.5%


Molecular Formula:

C36H56O8


TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6).


1.  Rebois, R.V. and Patel, J. (1985) J Biol Chem 260, 8026-31.

2.  Blumberg, P.M. (1980) Crit Rev Toxicol 8, 153-97.

3.  Fürstenberger, G. et al. (1981) Proc Natl Acad Sci U S A 78, 7722-6.

4.  Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5357-61.

5.  Lee, H.W. et al. (2002) J Biol Chem 277, 32624-31.

6.  Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5362-5.



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