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8132S 500 µg $209.00
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Western blot analysis of extracts from LNCaP cells, untreated (-) or treated with 17-AAG (1 μM, 24 hr), using Akt (pan) (11E7) Rabbit mAb #4685 (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).

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Chemical structure of 17-AAG (17-Demethoxy-17-allyaminogeldanmycin)

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Product Usage Information

17-AAG is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 500 μg in 853.7 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 0.1-1 μM for 4-24 hours. Soluble in DMSO at 150 mg/mL; soluble in ethanol at 5 mg/mL. Maximum solubility in plain water estimated to be about 20-50 μM.


Storage: Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

585.70 g/mol


Purity:

>99%


Molecular Formula:

C31H43N3O8


17-AAG is a semi-synthetic derivative of geldanamycin, demonstrating greater stability than its parent compound (1). It binds specifically to heat shock protein HSP90 in a manner similar to geldanamycin, but with weaker binding (1). Through specific binding with HSP90, 17-AAG has been shown to decrease levels of many proteins including androgen receptor (AR), HER2, and Akt, while increasing levels of HSP90 in prostate cancer cell lines (2). 17-AAG binds to a conserved pocket in the HSP90 family of chaperone proteins and this occupation causes the degradation of several signaling proteins (2).


1.  Schulte, T.W. and Neckers, L.M. (1998) Cancer Chemother Pharmacol 42, 273-9.

2.  Solit, D.B. et al. (2002) Clin Cancer Res 8, 986-93.



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