News from the Bench
Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.
CST Antibody Performance Guarantee
To Purchase # 9886S
Find answers on our FAQs page.
PTM information and tools available.
Western blot analysis of extracts from HT-29 cells treated with Docetaxel (100 ng/ml) for the indicated times using Cyclin B1 Antibody #4138 (upper) or Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb #3079 (lower).Learn more about how we get our images
Gallery: Docetaxel #9886
Docetaxel is supplied as 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1 ml DMSO to make a 1 mg/ml stock solution. The suggested working concentration is 100 ng/ml in tissue culture medium. Treat cells for 4-24 hours. Store solution at -20ºC.Storage: Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Docetaxel belongs to the taxane family of antitumor and antileukemic agents (1). A microtubule-stabilizing agent, docetaxel acts as an inhibitor of cellular functions that require microtubule dynamics including cell division, and is now in use as a treatment for breast, prostate and lung cancer (2).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.