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9902
Calyculin A
Activators & Inhibitors
Chemical Modulators

Calyculin A #9902

Citations (0)
Western blot analysis of extracts from Jurkat cells, untreated or treated with Calyculin (0.1 µM for 45 minutes prior to lysis), using P-Thr-Polyclonal #9381. Proteins were separated by 2D electrophoresis prior to blotting.
Western blot analysis of extracts from A431 cells treated with varied concentrations of Okadaic Acid or Calyculin A (30 minutes) using P-Thr-Polyclonal #9381.

Product Usage Information

Calyculin A is supplied as a lyophilized clear film. For 10 µM stock, reconstitute in 1 ml DMSO. Store in aliquots at -20ºC in the dark. Treat cells with 50-100 nM calyculin A for 5-30 minutes. Store in aliquots tightly sealed (unopened) at -20ºC in the dark. See MSDS.

This compound is sold only for use in extremely dilute solutions for biological research. No other use is intended and any other use involves substantial hazards. This compound should never be handled in powder or aerosol form or in any other form susceptible to uncontrolled release in the laboratory, even in very small quantities.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, continue to store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Calyculin A is a more potent phosphatase inhibitor than Okadaic acid (2). As shown by Western blot, treatment of cells with 100 nM Calyculin A for 30 minutes induces threonine phosphorylation, detected by Phospho-Threonine-Polyclonal Antibody #9381. IC50 values for inhibitory activity against PP1 are approximately 2 nM. IC50 values for inhibitory activity against PP2A are approximately 0.5 -1.0 nM.
Molecular Weight 1009.17 g/mol
Purity >98%
Molecular Formula C50H81N4O15P
CAS 101932-71-2
Solubility Soluble in DMSO at 50mM and EtOH at 1mg/ml.

Background

Calyculin A inhibits the activity of protein phosphatases PP1 and PP2A (1,2). Unlike Okadaic acid, which reduces PP2A activity but has little effect on PP1 activity, Calyculin A inhibits both phosphatases (1). Neither Calyculin A nor Okadaic acid inhibit acid or alkaline phosphatases or phospho- tyrosine protein phosphatases (2).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 9902
Cat. # Size Qty. Price
9902S
10 µg