News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.

Subscribe

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Western blot analysis of extracts from untreated, wortmannin and/or LY294002 treated Jurkat cells, using Phospho-Akt (Ser473) Antibody #9271 (A), Phospho-Akt (Thr308) Antibody #9275 (B), Akt Antibody #9272 (C) and Phospho-GSK-3alpha/beta (Ser21/9) Antibody #9331 (D).

Learn more about how we got this image
Image

Product Usage Information

For 2 mM stock, dissolve 1 mg in 1.16 ml DMSO. For experiments with cultured cells, CST recommends treating with wortmannin at concentrations between 0.2 µM and 1 µM, for one hour prior to, and for the duration of, the stimulation. See MSDS for further information.

Precautions: This compound is only sold for use in extremely dilute solutions for biological research. No other use is intended and any other use involves substantial hazards. This compound should never be handled in powder or aerosol form or in any other form susceptible to uncontrolled release in the laboratory, even in very small quantities.


Storage: Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

428.4 g/mol


Purity:

>99%


Molecular Formula:

C23H24O8


Wortmannin is a very potent, specific and direct inhibitor of PI3 kinase, originally derived from fungus (1,2). The inhibition is irreversible and noncompetitive. Wortmannin does not inhibit PI4 kinase, protein kinase C or protein tyrosine kinase (3).


1.  Nakanishi, S. et al. (1992) J. Biol. Chem. 267, 2157-2163.

2.  Arcaro, A. and Wymann, M.P. (1993) Biochem. J. 296, 297-301.

3.  Powis, G. et al. (1994) Cancer Res. 54, 5241-5248.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.