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Western blot analysis of extracts from HeLa cells, untreated or Staurosporine-treated (3 hours), showing PARP cleavage as evidence of induction of apoptosis, using Cleaved PARP Antibody #9541 (upper) or PARP Antibody #9542 (lower).

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Chemical structure of Staurosporine.

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Product Usage Information

Dissolve the lyophilized alkaloid in DMSO or Methanol. For a 1 mM solution, dissolve in 0.536 mL.


Storage: Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

466.53 g/mol


Purity:

>99%


Molecular Formula:

C28H26N4O3


Staurosporine is an alkaloid isolated from the culture broth of Streptomyces staurosporesa. It is a potent, cell permeable protein kinase C inhibitor with an IC50 of 0.7 nM. At higher concentration (1-20 nM), staurosporine also inhibits other kinases such as PKA, PKG, CAMKII and Myosin light chain kinase (MLCK) (1). At 50-100 nM, it is a functional neurotrophin agonist, promoting neurite outgrowth in neuroblastoma, pheochromocytoma and brain primary neuronal cultures. At 0.2- 1 μM, staurosporine induces cell apoptosis (2,3).


1.  Ruegg, U.T. and Burgess, G.M. (1989) Trends Pharmacol. Sci. 10, 218-220.

2.  Couldwell , W. T. et al. (1994) FEBS Lett. 345, 43-46.

3.  Yue, T. L. et al. (1998) J. Mol. Cell. Cardiol. 30, 495-507.



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