Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Thanksgiving. We will reopen on Monday, December 1st.

Thank you for your patience.

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

REACTIVITY MW (kDa)

Figure 1. Target map of the PathScan® Intracellular Signaling Array Kit (Chemiluminescent Readout) #7323.

Learn more about how we get our images

Figure 2. MCF7 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (left panel) or treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (100 ng/ml, 20 min). Cell extracts were prepared and analyzed using the PathScan® Intracellular Signaling Array Kit (Chemiluminescent Readout) #7323. Images were acquired by briefly exposing the slide to standard chemiluminescent film.

Learn more about how we get our images

Figure 3. HT-29 cells were grown to 80% confluency and then either untreated (left panel) or UV-irradiated and allowed to recover for 60 min (right panel). Cell extracts were prepared and analyzed using the PathScan® Intracellular Signaling Array Kit (Chemiluminescent Readout) #7323. Images were acquired by briefly exposing the slide to standard chemiluminescent film.

Learn more about how we get our images

Figure 4. HeLa cells were grown to 90% confluency and then either untreated (upper panel) or treated with Staurosporine #9953 (1 μM, 3.5 hr; lower panel). Cell extracts were prepared and analyzed using the PathScan® Intracellular Signaling Array Kit (Chemiluminescent Readout) #7323. Images were acquired by briefly exposing the slide to standard chemiluminescent film.

Learn more about how we get our images
Image
Product Includes Quantity
Array Slides - Intracellular Signaling Array Kit 2 Ea
16-Well Gasket 2 Ea
Sealing Tape 2 sheets
Chemiluminescent Development Folder
20X Array Wash Buffer 15 ml
Array Blocking Buffer 5 ml
Array Diluent Buffer 15 ml
Detection Ab Cocktail (10X) - Intracellular Signaling Array Kit 300 µl
HRP-Linked Streptavidin (10X) 300 µl
20X LumiGLO Reagent A 5 ml
20X Peroxide Reagent B 5 ml
PathScan® Sandwich ELISA Lysis Buffer (1X) 7018 30 ml

Product Usage Information

Storage: Kit should be stored at 4°C with the exception of Lysis Buffer, which is stored at –20°C (packaged separately).

Product Description

The PathScan® Intracellular Signaling Array Kit (Chemiluminescent Readout) is a slide-based antibody array founded upon the sandwich immunoassay principle. The array kit allows for the simultaneous detection of 18 important and well-characterized signaling molecules when phosphorylated or cleaved. Target-specific capture antibodies have been spotted in duplicate onto nitrocellulose-coated glass slides. Each kit contains two 16-pad slides, allowing the user to test up to 32 samples and generate 576 data points in a single experiment. Cell lysate is incubated on the slide followed by a biotinylated detection antibody cocktail. Streptavidin-conjugated HRP and LumiGLO® Reagent are then used to visualize the bound detection antibody by chemiluminescence. An image of the slide can be captured with either a digital imaging system or standard chemiluminescent film. The image can be analyzed visually or the spot intensities quantified using array analysis software.


Specificity / Sensitivity

PathScan® Intracellular Signaling Array Kit (Chemiluminescent Readout) detects the indicated cellular proteins and signaling nodes only when phosphorylated or cleaved at the specified residues. (see Array Target Map). No significant cross-reactivity has been observed between targets. This kit is optimized for cell lysates diluted to a total protein concentration between 0.2 and 1 mg/ml (see kit protocol). All capture antibodies have been validated for human and mouse-derived samples.


Phosphorylation and proteolysis are two widespread covalent post-translational modifications that represent important regulatory mechanisms in biology. Detection of these modifications on a set of cellular proteins playing a well-understood role in cell biology can provide a broad snapshot of intracellular signaling.

The MAPK/Erk cascade is one of the best characterized and widely studied signaling modules. It is involved in a broad range of cellular processes such as proliferation, differentiation, and motility. MAPK/Erk is activated by a wide range of extracellular signals including growth factors, cytokines, hormones, and neurotransmitters. It is activated by dual phosphorylation at Thr202 and Tyr204 by the dual specificity kinases MEK1 and MEK2.

p38 and JNK MAPKs are core components of two additional structurally related signal transduction modules. p38 and JNK are activated through a similar dual phosphorylation mechanism by various MAPK kinases in response to pro-inflammatory cytokines, stressful conditions, or genotoxicity.

Stat1 and Stat3 are important signaling molecules that are involved in immunity and inflammation and can be activated by a variety of cytokines or growth factors. Stat1 and Stat3 are phosphorylated at Tyr701 or Tyr705, respectively, by cytokine receptor-tethered tyrosine kinases of the Jak family or, in some cases, by other tyrosine kinases such as Src.

Akt is a protein kinase generally activated in response to growth factor stimulation that transmits growth and survival signals. Phosphorylation of Akt at Ser473 and Thr308 by TORC2 complex and PDK1, respectively, are reliable predictors of Akt activation. Phosphorylation of PRAS40 at Thr246 by Akt relieves PRAS40 inhibition of TORC1. Akt phosphorylation of the pro-apoptotic protein Bad at Ser112 and the multifunctional kinase GSK-3β at Ser9 inhibits their activity and promotes cell survival.

mTOR is an important signaling hub that is a major component of two macromolecular complexes, TORC1 and TORC2. mTOR is phosphorylated at Ser2448 and integrates growth factor signaling and nutrient availability, thus playing an important role in cell growth and homeostasis. mTORC1 phosphorylates p70 S6 Kinase at Thr389, leading to kinase activation and cell cycle progression. The S6 ribosomal protein is found downstream of p70 S6 Kinase and its phosphorylation at Ser235/236 reflects mTOR pathway activation and predicts cell cycle progression.

AMPK is an energy sensor that is activated by phosphorylation at Thr172 in response to elevated AMP levels. AMPK regulates fatty acid metabolism, as well as modulates protein synthesis and cell growth.

HSP27 is a mediator of cell stress that confers resistance to adverse environmental change. HSP27 is phosphorylated at Ser78 within the p38 MAPK pathway.

p53 plays an important role in cellular response to DNA damage and other genomic aberrations. Phosphorylation of p53 at Ser15 by ATM/ATR or DNA-PK in response to DNA damage leads to its stabilization and accumulation.

Caspase-3 is a critical executor of apoptosis. Caspase-3 is activated by endoproteolytic cleavage at Asp175 and exerts its pro-apoptotic activity through cleavage of multiple cellular targets. PARP, an enzyme that is involved in DNA repair, is one of the main substrates of activated caspase-3. Cleavage at Asp214 leads to PARP inactivation. Increased levels of cleaved caspase-3 and cleaved PARP are reliable indicators of apoptosis.


1.  Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.

2.  Boatright, K.M. and Salvesen, G.S. (2003) Curr Opin Cell Biol 15, 725-31.

3.  Manning, B.D. and Cantley, L.C. (2007) Cell 129, 1261-74.

4.  Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.

5.  Keshet, Y. and Seger, R. (2010) Methods Mol Biol 661, 3-38.

6.  Manning, G. et al. (2002) Science 298, 1912-34.

7.  Huang, J. and Manning, B.D. (2009) Biochem Soc Trans 37, 217-22.

8.  Cuadrado, A. and Nebreda, A.R. (2010) Biochem J 429, 403-17.

9.  Brognard, J. and Hunter, T. (2011) Curr Opin Genet Dev 21, 4-11.

10.  Hunter, T. (2009) Curr Opin Cell Biol 21, 140-6.

11.  Kurokawa, M. and Kornbluth, S. (2009) Cell 138, 838-54.

12.  Hardie, D.G. et al. (2012) Nat Rev Mol Cell Biol 13, 251-62.

13.  Shi, Y. (2004) Cell 117, 855-8.

14.  Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.

15.  Boatright, K.M. and Salvesen, G.S. (2003) Curr Opin Cell Biol 15, 725-31.

16.  Manning, B.D. and Cantley, L.C. (2007) Cell 129, 1261-74.

17.  Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.

18.  Keshet, Y. and Seger, R. (2010) Methods Mol Biol 661, 3-38.

19.  Manning, G. et al. (2002) Science 298, 1912-34.

20.  Huang, J. and Manning, B.D. (2009) Biochem Soc Trans 37, 217-22.

21.  Cuadrado, A. and Nebreda, A.R. (2010) Biochem J 429, 403-17.

22.  Brognard, J. and Hunter, T. (2011) Curr Opin Genet Dev 21, 4-11.

23.  Hunter, T. (2009) Curr Opin Cell Biol 21, 140-6.

24.  Kurokawa, M. and Kornbluth, S. (2009) Cell 138, 838-54.

25.  Hardie, D.G. et al. (2012) Nat Rev Mol Cell Biol 13, 251-62.

26.  Shi, Y. (2004) Cell 117, 855-8.


Entrez-Gene Id 207, 208, 10000, 5562, 5563, 572, 836, 5595, 5594, 2932, 3315, 2475, 142, 84335, 1432, 5600, 5603, 6300, 7157, 6198, 5599, 6772, 6774, 6194
Swiss-Prot Acc. P31749, P31751, Q9Y243, Q13131, P54646, Q92934, P42574, P27361, P28482, P49841, P04792, P42345, P09874, Q96B36, Q16539, Q15759, O15264, P53778, P04637, P23443, P45983, P42224, P40763, P62753

Protein Specific References

Germack R and Dickenson JM (2000) Br J Pharmacol 130, 867–74

Wick MJ et al. (2000) J Biol Chem 275, 40400–6

Rane MJ et al. (2001) J Biol Chem 276, 3517–23

Guizzetti M and Costa LG (2001) Neuroreport 12, 1639–42

Brognard J et al. (2001) Cancer Res 61, 3986–97

Maira SM et al. (2001) Science 294, 374–80

Schönherr E et al. (2001) J Biol Chem 276, 40687–92

Hill MM et al. (2001) J Biol Chem 276, 25643–6

Dhawan P et al. (2002) Cancer Res 62, 7335–42

Conus NM et al. (2002) J Biol Chem 277, 38021–8

Sano H et al. (2002) J Biol Chem 277, 19439–47

Egawa K et al. (2002) J Biol Chem 277, 38863–9

Kisseleva MV et al. (2002) J Biol Chem 277, 6266–72

Barry FA and Gibbins JM (2002) J Biol Chem 277, 12874–8

Ikonomov OC et al. (2002) Endocrinology 143, 4742–54

Rani MR et al. (2002) J Biol Chem 277, 38456–61

Ho R et al. (2002) Cancer Res 62, 6462–6

Wan X and Helman LJ (2003) Oncogene 22, 8205–11

Fukuda T et al. (2003) J Biol Chem 278, 51324–33

Kim HH et al. (2003) FASEB J 17, 2163–5

Min YH et al. (2004) Cancer Res 64, 5225–31

Tazzari PL et al. (2004) Br J Haematol 126, 675–81

Matsuzaki H et al. (2004) Biochemistry 43, 4284–93

Wolfrum S et al. (2004) Arterioscler Thromb Vasc Biol 24, 1842–7

Kaneko Y et al. (2004) J Cell Sci 117, 407–15

Esfandiarei M et al. (2004) J Virol 78, 4289–98

Baudhuin LM et al. (2004) FASEB J 18, 341–3

Dietze EC et al. (2004) Oncogene 23, 3851–62

Wu T et al. (2004) Mol Cancer Ther 3, 299–307

Honjo S et al. (2005) DNA Cell Biol 24, 141–7

Karlsson HK et al. (2005) Diabetes 54, 1459–67

Viniegra JG et al. (2005) J Biol Chem 280, 4029–36

Le XF et al. (2005) J Biol Chem 280, 2092–104

Smith E and Frenkel B (2005) J Biol Chem 280, 2388–94

Edwards LA et al. (2005) Oncogene 24, 3596–605

Karlsson HK et al. (2005) Diabetes 54, 1692–7

Kippenberger S et al. (2005) J Biol Chem 280, 3060–7

Jung HS et al. (2005) Mol Endocrinol 19, 2748–59

Khundmiri SJ et al. (2006) Am J Physiol Cell Physiol 291, C1247–57

Hers I and (2007) Blood 110, 4243–52

Ananthanarayanan B et al. (2007) J Biol Chem 282, 36634–41

Zunder ER et al. (2008) Cancer Cell 14, 180–92

Grenegård M et al. (2008) J Biol Chem 283, 18493–504

Abubaker J et al. (2009) Mol Cancer 8, 51

Chen PL and Easton AS (2011) Curr Neurovasc Res 8, 14–24

Van Aller GS et al. (2011) Biochem Biophys Res Commun 406, 194–9

Uesugi A et al. (2011) Cancer Res 71, 5765–78

Ou YH et al. (2011) Mol Cell 41, 458–70

Wang S et al. (2012) PLoS One 7, e37427

Glidden EJ et al. (2012) J Biol Chem 287, 581–8

Shih MC et al. (2012) Oncogene 31, 2389–400

Misra UK and Pizzo SV (2012) J Cell Biochem 113, 1488–500

Germack R and Dickenson JM (2000) Br J Pharmacol 130, 867–74

Wick MJ et al. (2000) J Biol Chem 275, 40400–6

Rane MJ et al. (2001) J Biol Chem 276, 3517–23

Guizzetti M and Costa LG (2001) Neuroreport 12, 1639–42

Brognard J et al. (2001) Cancer Res 61, 3986–97

Maira SM et al. (2001) Science 294, 374–80

Schönherr E et al. (2001) J Biol Chem 276, 40687–92

Hill MM et al. (2001) J Biol Chem 276, 25643–6

Dhawan P et al. (2002) Cancer Res 62, 7335–42

Conus NM et al. (2002) J Biol Chem 277, 38021–8

Sano H et al. (2002) J Biol Chem 277, 19439–47

Egawa K et al. (2002) J Biol Chem 277, 38863–9

Kisseleva MV et al. (2002) J Biol Chem 277, 6266–72

Barry FA and Gibbins JM (2002) J Biol Chem 277, 12874–8

Ikonomov OC et al. (2002) Endocrinology 143, 4742–54

Rani MR et al. (2002) J Biol Chem 277, 38456–61

Ho R et al. (2002) Cancer Res 62, 6462–6

Wan X and Helman LJ (2003) Oncogene 22, 8205–11

Fukuda T et al. (2003) J Biol Chem 278, 51324–33

Kim HH et al. (2003) FASEB J 17, 2163–5

Min YH et al. (2004) Cancer Res 64, 5225–31

Tazzari PL et al. (2004) Br J Haematol 126, 675–81

Matsuzaki H et al. (2004) Biochemistry 43, 4284–93

Wolfrum S et al. (2004) Arterioscler Thromb Vasc Biol 24, 1842–7

Kaneko Y et al. (2004) J Cell Sci 117, 407–15

Esfandiarei M et al. (2004) J Virol 78, 4289–98

Baudhuin LM et al. (2004) FASEB J 18, 341–3

Dietze EC et al. (2004) Oncogene 23, 3851–62

Wu T et al. (2004) Mol Cancer Ther 3, 299–307

Honjo S et al. (2005) DNA Cell Biol 24, 141–7

Karlsson HK et al. (2005) Diabetes 54, 1459–67

Viniegra JG et al. (2005) J Biol Chem 280, 4029–36

Le XF et al. (2005) J Biol Chem 280, 2092–104

Smith E and Frenkel B (2005) J Biol Chem 280, 2388–94

Edwards LA et al. (2005) Oncogene 24, 3596–605

Karlsson HK et al. (2005) Diabetes 54, 1692–7

Kippenberger S et al. (2005) J Biol Chem 280, 3060–7

Jung HS et al. (2005) Mol Endocrinol 19, 2748–59

Khundmiri SJ et al. (2006) Am J Physiol Cell Physiol 291, C1247–57

Hers I and (2007) Blood 110, 4243–52

Ananthanarayanan B et al. (2007) J Biol Chem 282, 36634–41

Zunder ER et al. (2008) Cancer Cell 14, 180–92

Grenegård M et al. (2008) J Biol Chem 283, 18493–504

Abubaker J et al. (2009) Mol Cancer 8, 51

Chen PL and Easton AS (2011) Curr Neurovasc Res 8, 14–24

Van Aller GS et al. (2011) Biochem Biophys Res Commun 406, 194–9

Uesugi A et al. (2011) Cancer Res 71, 5765–78

Ou YH et al. (2011) Mol Cell 41, 458–70

Wang S et al. (2012) PLoS One 7, e37427

Glidden EJ et al. (2012) J Biol Chem 287, 581–8

Shih MC et al. (2012) Oncogene 31, 2389–400

Misra UK and Pizzo SV (2012) J Cell Biochem 113, 1488–500

Germack R and Dickenson JM (2000) Br J Pharmacol 130, 867–74

Wick MJ et al. (2000) J Biol Chem 275, 40400–6

Rane MJ et al. (2001) J Biol Chem 276, 3517–23

Guizzetti M and Costa LG (2001) Neuroreport 12, 1639–42

Brognard J et al. (2001) Cancer Res 61, 3986–97

Maira SM et al. (2001) Science 294, 374–80

Schönherr E et al. (2001) J Biol Chem 276, 40687–92

Hill MM et al. (2001) J Biol Chem 276, 25643–6

Dhawan P et al. (2002) Cancer Res 62, 7335–42

Conus NM et al. (2002) J Biol Chem 277, 38021–8

Sano H et al. (2002) J Biol Chem 277, 19439–47

Egawa K et al. (2002) J Biol Chem 277, 38863–9

Kisseleva MV et al. (2002) J Biol Chem 277, 6266–72

Barry FA and Gibbins JM (2002) J Biol Chem 277, 12874–8

Ikonomov OC et al. (2002) Endocrinology 143, 4742–54

Rani MR et al. (2002) J Biol Chem 277, 38456–61

Ho R et al. (2002) Cancer Res 62, 6462–6

Wan X and Helman LJ (2003) Oncogene 22, 8205–11

Fukuda T et al. (2003) J Biol Chem 278, 51324–33

Kim HH et al. (2003) FASEB J 17, 2163–5

Min YH et al. (2004) Cancer Res 64, 5225–31

Tazzari PL et al. (2004) Br J Haematol 126, 675–81

Matsuzaki H et al. (2004) Biochemistry 43, 4284–93

Wolfrum S et al. (2004) Arterioscler Thromb Vasc Biol 24, 1842–7

Kaneko Y et al. (2004) J Cell Sci 117, 407–15

Esfandiarei M et al. (2004) J Virol 78, 4289–98

Baudhuin LM et al. (2004) FASEB J 18, 341–3

Dietze EC et al. (2004) Oncogene 23, 3851–62

Wu T et al. (2004) Mol Cancer Ther 3, 299–307

Honjo S et al. (2005) DNA Cell Biol 24, 141–7

Karlsson HK et al. (2005) Diabetes 54, 1459–67

Viniegra JG et al. (2005) J Biol Chem 280, 4029–36

Le XF et al. (2005) J Biol Chem 280, 2092–104

Smith E and Frenkel B (2005) J Biol Chem 280, 2388–94

Edwards LA et al. (2005) Oncogene 24, 3596–605

Karlsson HK et al. (2005) Diabetes 54, 1692–7

Kippenberger S et al. (2005) J Biol Chem 280, 3060–7

Jung HS et al. (2005) Mol Endocrinol 19, 2748–59

Khundmiri SJ et al. (2006) Am J Physiol Cell Physiol 291, C1247–57

Hers I and (2007) Blood 110, 4243–52

Ananthanarayanan B et al. (2007) J Biol Chem 282, 36634–41

Zunder ER et al. (2008) Cancer Cell 14, 180–92

Grenegård M et al. (2008) J Biol Chem 283, 18493–504

Abubaker J et al. (2009) Mol Cancer 8, 51

Chen PL and Easton AS (2011) Curr Neurovasc Res 8, 14–24

Van Aller GS et al. (2011) Biochem Biophys Res Commun 406, 194–9

Uesugi A et al. (2011) Cancer Res 71, 5765–78

Ou YH et al. (2011) Mol Cell 41, 458–70

Wang S et al. (2012) PLoS One 7, e37427

Glidden EJ et al. (2012) J Biol Chem 287, 581–8

Shih MC et al. (2012) Oncogene 31, 2389–400

Misra UK and Pizzo SV (2012) J Cell Biochem 113, 1488–500

Johnson AL et al. (2001) Biol Reprod 64, 1566–74

Zhang M and Riedel H (2009) J Cell Biochem 107, 65–75

Johnson AL et al. (2001) Biol Reprod 64, 1566–74

Zhang M and Riedel H (2009) J Cell Biochem 107, 65–75

Johnson AL et al. (2001) Biol Reprod 64, 1566–74

Zhang M and Riedel H (2009) J Cell Biochem 107, 65–75

Moon EY and Lerner A (2003) Blood 101, 4122–30

Rice PL et al. (2003) Cancer Res 63, 616–20

Zhang B et al. (2004) Mol Cell Biol 24, 6205–14

Hui L et al. (2005) J Biol Chem 280, 35829–35

Xu RH et al. (2005) Cancer Res 65, 613–21

Li YY et al. (2006) Cancer Res 66, 6741–7

Polzien L et al. (2009) J Biol Chem 284, 28004–20

Chen J et al. (2009) Oncogene 28, 2581–92

Ye DZ et al. (2011) PLoS One 6, e27637

Kumar JK et al. (2011) Int J Biochem Cell Biol 43, 594–603

Polzien L et al. (2011) J Biol Chem 286, 17934–44

Marchion DC et al. (2011) Clin Cancer Res 17, 6356–66

Xu D et al. (2011) Carcinogenesis 32, 488–95

Moon EY and Lerner A (2003) Blood 101, 4122–30

Rice PL et al. (2003) Cancer Res 63, 616–20

Zhang B et al. (2004) Mol Cell Biol 24, 6205–14

Hui L et al. (2005) J Biol Chem 280, 35829–35

Xu RH et al. (2005) Cancer Res 65, 613–21

Li YY et al. (2006) Cancer Res 66, 6741–7

Polzien L et al. (2009) J Biol Chem 284, 28004–20

Chen J et al. (2009) Oncogene 28, 2581–92

Ye DZ et al. (2011) PLoS One 6, e27637

Kumar JK et al. (2011) Int J Biochem Cell Biol 43, 594–603

Polzien L et al. (2011) J Biol Chem 286, 17934–44

Marchion DC et al. (2011) Clin Cancer Res 17, 6356–66

Xu D et al. (2011) Carcinogenesis 32, 488–95

Syme CA et al. (2005) J Biol Chem 280, 11281–8

Syme CA et al. (2005) J Biol Chem 280, 11281–8

Li Z et al. (2001) J Biol Chem 276, 42226–32

Li Z et al. (2001) J Biol Chem 276, 42226–32

Butt E et al. (2001) J Biol Chem 276, 7108–13

Benn SC et al. (2002) Neuron 36, 45–56

Rane MJ et al. (2003) J Biol Chem 278, 27828–35

Butt E et al. (2001) J Biol Chem 276, 7108–13

Benn SC et al. (2002) Neuron 36, 45–56

Rane MJ et al. (2003) J Biol Chem 278, 27828–35

Ekim B et al. (2011) Mol Cell Biol 31, 2787–801

Ekim B et al. (2011) Mol Cell Biol 31, 2787–801

Kauppinen TM et al. (2006) Proc Natl Acad Sci U S A 103, 7136–41

Wang L et al. (2008) J Biol Chem 283, 15619–27

Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94.

Relou IA et al. (2003) J Biol Chem 278, 32638–44

Pillai VB et al. (2011) Mol Cell Biol 31, 2349–63

Ando K et al. (2011) J Biol Chem 286, 7619–28

Chehab NH et al. (1999) Proc Natl Acad Sci U S A 96, 13777–82

Buschmann T et al. (2000) Cancer Res 60, 896–900

Chehab NH et al. (2000) Genes Dev 14, 278–88

Persons DL et al. (2000) J Biol Chem 275, 35778–85

Vaziri H et al. (2001) Cell 107, 149–59

Minamoto T et al. (2001) Oncogene 20, 3341–7

Stewart ZA et al. (2001) Oncogene 20, 113–24

Bean LJ and Stark GR (2001) Oncogene 20, 1076–84

Xie S et al. (2001) J Biol Chem 276, 43305–12

Xie S et al. (2001) J Biol Chem 276, 36194–9

Kim SJ et al. (2002) J Biol Chem 277, 33501–8

Bulavin DV et al. (2002) Nat Genet 31, 210–5

Shono T et al. (2002) Cancer Res 62, 1069–76

Adamson AW et al. (2002) J Biol Chem 277, 38222–9

Hase H et al. (2002) J Biol Chem 277, 46950–8

Bischof O et al. (2002) EMBO J 21, 3358–69

Qin JZ et al. (2002) Oncogene 21, 2991–3002

Hofmann TG et al. (2002) Nat Cell Biol 4, 1–10

Chouinard N et al. (2002) Biochem J 365, 133–45

Shiseki M et al. (2003) Cancer Res 63, 2373–8

Chen K et al. (2003) J Biol Chem 278, 39527–33

Sengupta S et al. (2003) EMBO J 22, 1210–22

Urban G et al. (2003) J Biol Chem 278, 9747–53

Oguchi K et al. (2003) Blood 101, 3622–7

Hideshima T et al. (2003) Blood 101, 1530–4

Lindström MS and Wiman KG (2003) Oncogene 22, 4993–5005

Hofseth LJ et al. (2003) Proc Natl Acad Sci U S A 100, 143–8

Wang C and Chen J (2003) J Biol Chem 278, 2066–71

Matsuoka M et al. (2003) Environ Health Perspect 111, 509–12

Yanamadala S and Ljungman M (2003) Mol Cancer Res 1, 747–54

Möller A et al. (2003) Cancer Res 63, 4310–4

Goudelock DM et al. (2003) J Biol Chem 278, 29940–7

Louria-Hayon I et al. (2003) J Biol Chem 278, 33134–41

Tritarelli A et al. (2004) Mol Biol Cell 15, 3751–7

Mroz RM et al. (2004) Am J Respir Cell Mol Biol 30, 564–8

Rui Y et al. (2004) EMBO J 23, 4583–94

Li Y et al. (2004) Oncogene 23, 7355–65

Vaghefi H and Neet KE (2004) Oncogene 23, 8078–87

Nair VD et al. (2004) J Biol Chem 279, 27494–501

Takagi M et al. (2004) Blood 103, 283–90

Ito K et al. (2004) Cancer Res 64, 1071–8

Koutsodontis G and Kardassis D (2004) Oncogene 23, 9190–200

Jackson MW et al. (2004) Oncogene 23, 4477–87

Dohoney KM et al. (2004) Oncogene 23, 49–57

Thompson T et al. (2004) J Biol Chem 279, 53015–22

Yeh PY et al. (2004) Oncogene 23, 3580–8

Komiyama S et al. (2004) Biochem Biophys Res Commun 323, 816–22

Soubeyrand S et al. (2004) Eur J Biochem 271, 3776–84

Ou YH et al. (2005) Mol Biol Cell 16, 1684–95

Feki A et al. (2005) Oncogene 24, 3726–36

Li Z et al. (2005) J Biol Chem 280, 16843–50

Di Stefano V et al. (2005) Oncogene 24, 5431–42

Hershko T et al. (2005) Cell Death Differ 12, 377–83

Mayo LD et al. (2005) J Biol Chem 280, 25953–9

Wesierska-Gadek J et al. (2005) Mol Cancer Ther 4, 113–24

Wang L et al. (2005) Oncogene 24, 3020–7

Zhao Y et al. (2006) Mol Cell Biol 26, 2782–90

Gresko E et al. (2006) EMBO J 25, 1883–94

Ichwan SJ et al. (2006) Oncogene 25, 1216–24

Knights CD et al. (2006) J Cell Biol 173, 533–44

Moiseeva O et al. (2006) Mol Biol Cell 17, 1583–92

Zeng PY and Berger SL (2006) Cancer Res 66, 10701–8

Li AG et al. (2006) Mol Cell 23, 575–87

Yoshida K et al. (2006) J Biol Chem 281, 5734–40

Li DW et al. (2006) Oncogene 25, 3006–22

Fraser M et al. (2006) Oncogene 25, 2203–12

Paulsen MT et al. (2006) Mol Cancer 5, 25

Singh K et al. (2007) PLoS One 2, e660

Nag A et al. (2007) J Biol Chem 282, 8812–20

Li HH et al. (2007) EMBO J 26, 402–11

Li Q et al. (2007) Cancer Res 67, 66–74

Taira N et al. (2007) Mol Cell 25, 725–38

Lambrot R et al. (2007) J Clin Endocrinol Metab 92, 2632–9

Ivanov GS et al. (2007) Mol Cell Biol 27, 6756–69

Nakanishi M et al. (2007) J Biol Chem 282, 22993–3004

Liu Y et al. (2007) J Biol Chem 282, 2505–11

Derheimer FA et al. (2007) Proc Natl Acad Sci U S A 104, 12778–83

Lee JH et al. (2007) J Cell Sci 120, 2259–71

Mantovani F et al. (2007) Nat Struct Mol Biol 14, 912–20

Wang H et al. (2008) J Biol Chem 283, 2564–74

Sun L et al. (2008) J Exp Clin Cancer Res 27, 35

Chang PC and Li M (2008) J Virol 82, 278–90

Lin T et al. (2008) Toxicology 247, 145–53

Chen JJ et al. (2008) J Immunol 180, 8030–9

Shouse GP et al. (2008) Mol Cell Biol 28, 448–56

Kitagawa M et al. (2008) Mol Cell 29, 217–31

Habold C et al. (2008) J Cell Mol Med 12, 607–21

Zhu H et al. (2008) Int J Cancer 123, 2741–9

Yamaguchi H et al. (2009) J Biol Chem 284, 11171–83

Wang Z et al. (2009) Pharm Res 26, 1140–8

Bar JK et al. (2009) Int J Gynecol Cancer 19, 1322–8

Nishimura T et al. (2009) J Biol Chem 284, 36442–52

Puca R et al. (2009) Mol Cancer 8, 85

Yadavilli S et al. (2009) J Biochem Mol Toxicol 23, 373–86

Baxter EW and Milner J (2010) J Neurooncol 97, 373–82

Fraser JA et al. (2010) J Biol Chem 285, 37773–86

Fraser JA et al. (2010) J Biol Chem 285, 37762–72

Shang X et al. (2010) Oncogene 29, 4938–46

Chen X et al. (2010) J Biol Chem 285, 12823–30

Kawano T et al. (2010) Int J Oncol 37, 787–95

Moehlenbrink J et al. (2010) Cancer Lett 292, 119–24

Venerando A et al. (2010) Cell Mol Life Sci 67, 1105–18

Puca R et al. (2010) Free Radic Biol Med 48, 1338–46

Marchenko ND et al. (2010) Cell Death Differ 17, 255–67

Aranha MM et al. (2011) PLoS One 6, e21396

Muñoz-Fontela C et al. (2011) Cell Cycle 10, 3701–5

Wu L et al. (2011) J Biol Chem 286, 2236–44

Ozeki C et al. (2011) J Biol Chem 286, 18251–60

Mellert HS et al. (2011) J Biol Chem 286, 4264–70

Valbuena A et al. (2011) PLoS One 6, e17320

Xu S et al. (2011) J Cardiovasc Pharmacol 58, 263–71

Seo SK et al. (2011) J Thorac Oncol 6, 1313–9

Savelyeva I and Dobbelstein M (2011) Oncogene 30, 865–75

Wu ZZ et al. (2011) J Cell Physiol 226, 2415–28

Smeenk L et al. (2011) PLoS One 6, e17574

Grison A et al. (2011) Proc Natl Acad Sci U S A 108, 17979–84

Gully CP et al. (2012) Proc Natl Acad Sci U S A 109, E1513–22

Wang H et al. (2012) DNA Repair (Amst) 11, 146–56

Xu S et al. (2013) Cell Res 23, 423–35

Chan C et al. (2013) Mol Cell Biol 33, 485–97

Thakur BK et al. (2013) Int J Cancer 132, 766–74

Yang Y et al. (2013) J Biol Chem 288, 529–39

Chehab NH et al. (1999) Proc Natl Acad Sci U S A 96, 13777–82

Buschmann T et al. (2000) Cancer Res 60, 896–900

Chehab NH et al. (2000) Genes Dev 14, 278–88

Persons DL et al. (2000) J Biol Chem 275, 35778–85

Vaziri H et al. (2001) Cell 107, 149–59

Minamoto T et al. (2001) Oncogene 20, 3341–7

Stewart ZA et al. (2001) Oncogene 20, 113–24

Bean LJ and Stark GR (2001) Oncogene 20, 1076–84

Xie S et al. (2001) J Biol Chem 276, 43305–12

Xie S et al. (2001) J Biol Chem 276, 36194–9

Kim SJ et al. (2002) J Biol Chem 277, 33501–8

Bulavin DV et al. (2002) Nat Genet 31, 210–5

Shono T et al. (2002) Cancer Res 62, 1069–76

Adamson AW et al. (2002) J Biol Chem 277, 38222–9

Hase H et al. (2002) J Biol Chem 277, 46950–8

Bischof O et al. (2002) EMBO J 21, 3358–69

Qin JZ et al. (2002) Oncogene 21, 2991–3002

Hofmann TG et al. (2002) Nat Cell Biol 4, 1–10

Chouinard N et al. (2002) Biochem J 365, 133–45

Shiseki M et al. (2003) Cancer Res 63, 2373–8

Chen K et al. (2003) J Biol Chem 278, 39527–33

Sengupta S et al. (2003) EMBO J 22, 1210–22

Urban G et al. (2003) J Biol Chem 278, 9747–53

Oguchi K et al. (2003) Blood 101, 3622–7

Hideshima T et al. (2003) Blood 101, 1530–4

Lindström MS and Wiman KG (2003) Oncogene 22, 4993–5005

Hofseth LJ et al. (2003) Proc Natl Acad Sci U S A 100, 143–8

Wang C and Chen J (2003) J Biol Chem 278, 2066–71

Matsuoka M et al. (2003) Environ Health Perspect 111, 509–12

Yanamadala S and Ljungman M (2003) Mol Cancer Res 1, 747–54

Möller A et al. (2003) Cancer Res 63, 4310–4

Goudelock DM et al. (2003) J Biol Chem 278, 29940–7

Louria-Hayon I et al. (2003) J Biol Chem 278, 33134–41

Tritarelli A et al. (2004) Mol Biol Cell 15, 3751–7

Mroz RM et al. (2004) Am J Respir Cell Mol Biol 30, 564–8

Rui Y et al. (2004) EMBO J 23, 4583–94

Li Y et al. (2004) Oncogene 23, 7355–65

Vaghefi H and Neet KE (2004) Oncogene 23, 8078–87

Nair VD et al. (2004) J Biol Chem 279, 27494–501

Takagi M et al. (2004) Blood 103, 283–90

Ito K et al. (2004) Cancer Res 64, 1071–8

Koutsodontis G and Kardassis D (2004) Oncogene 23, 9190–200

Jackson MW et al. (2004) Oncogene 23, 4477–87

Dohoney KM et al. (2004) Oncogene 23, 49–57

Thompson T et al. (2004) J Biol Chem 279, 53015–22

Yeh PY et al. (2004) Oncogene 23, 3580–8

Komiyama S et al. (2004) Biochem Biophys Res Commun 323, 816–22

Soubeyrand S et al. (2004) Eur J Biochem 271, 3776–84

Ou YH et al. (2005) Mol Biol Cell 16, 1684–95

Feki A et al. (2005) Oncogene 24, 3726–36

Li Z et al. (2005) J Biol Chem 280, 16843–50

Di Stefano V et al. (2005) Oncogene 24, 5431–42

Hershko T et al. (2005) Cell Death Differ 12, 377–83

Mayo LD et al. (2005) J Biol Chem 280, 25953–9

Wesierska-Gadek J et al. (2005) Mol Cancer Ther 4, 113–24

Wang L et al. (2005) Oncogene 24, 3020–7

Zhao Y et al. (2006) Mol Cell Biol 26, 2782–90

Gresko E et al. (2006) EMBO J 25, 1883–94

Ichwan SJ et al. (2006) Oncogene 25, 1216–24

Knights CD et al. (2006) J Cell Biol 173, 533–44

Moiseeva O et al. (2006) Mol Biol Cell 17, 1583–92

Zeng PY and Berger SL (2006) Cancer Res 66, 10701–8

Li AG et al. (2006) Mol Cell 23, 575–87

Yoshida K et al. (2006) J Biol Chem 281, 5734–40

Li DW et al. (2006) Oncogene 25, 3006–22

Fraser M et al. (2006) Oncogene 25, 2203–12

Paulsen MT et al. (2006) Mol Cancer 5, 25

Singh K et al. (2007) PLoS One 2, e660

Nag A et al. (2007) J Biol Chem 282, 8812–20

Li HH et al. (2007) EMBO J 26, 402–11

Li Q et al. (2007) Cancer Res 67, 66–74

Taira N et al. (2007) Mol Cell 25, 725–38

Lambrot R et al. (2007) J Clin Endocrinol Metab 92, 2632–9

Ivanov GS et al. (2007) Mol Cell Biol 27, 6756–69

Nakanishi M et al. (2007) J Biol Chem 282, 22993–3004

Liu Y et al. (2007) J Biol Chem 282, 2505–11

Derheimer FA et al. (2007) Proc Natl Acad Sci U S A 104, 12778–83

Lee JH et al. (2007) J Cell Sci 120, 2259–71

Mantovani F et al. (2007) Nat Struct Mol Biol 14, 912–20

Wang H et al. (2008) J Biol Chem 283, 2564–74

Sun L et al. (2008) J Exp Clin Cancer Res 27, 35

Chang PC and Li M (2008) J Virol 82, 278–90

Lin T et al. (2008) Toxicology 247, 145–53

Chen JJ et al. (2008) J Immunol 180, 8030–9

Shouse GP et al. (2008) Mol Cell Biol 28, 448–56

Kitagawa M et al. (2008) Mol Cell 29, 217–31

Habold C et al. (2008) J Cell Mol Med 12, 607–21

Zhu H et al. (2008) Int J Cancer 123, 2741–9

Yamaguchi H et al. (2009) J Biol Chem 284, 11171–83

Wang Z et al. (2009) Pharm Res 26, 1140–8

Bar JK et al. (2009) Int J Gynecol Cancer 19, 1322–8

Nishimura T et al. (2009) J Biol Chem 284, 36442–52

Puca R et al. (2009) Mol Cancer 8, 85

Yadavilli S et al. (2009) J Biochem Mol Toxicol 23, 373–86

Baxter EW and Milner J (2010) J Neurooncol 97, 373–82

Fraser JA et al. (2010) J Biol Chem 285, 37773–86

Fraser JA et al. (2010) J Biol Chem 285, 37762–72

Shang X et al. (2010) Oncogene 29, 4938–46

Chen X et al. (2010) J Biol Chem 285, 12823–30

Kawano T et al. (2010) Int J Oncol 37, 787–95

Moehlenbrink J et al. (2010) Cancer Lett 292, 119–24

Venerando A et al. (2010) Cell Mol Life Sci 67, 1105–18

Puca R et al. (2010) Free Radic Biol Med 48, 1338–46

Marchenko ND et al. (2010) Cell Death Differ 17, 255–67

Aranha MM et al. (2011) PLoS One 6, e21396

Muñoz-Fontela C et al. (2011) Cell Cycle 10, 3701–5

Wu L et al. (2011) J Biol Chem 286, 2236–44

Ozeki C et al. (2011) J Biol Chem 286, 18251–60

Mellert HS et al. (2011) J Biol Chem 286, 4264–70

Valbuena A et al. (2011) PLoS One 6, e17320

Xu S et al. (2011) J Cardiovasc Pharmacol 58, 263–71

Seo SK et al. (2011) J Thorac Oncol 6, 1313–9

Savelyeva I and Dobbelstein M (2011) Oncogene 30, 865–75

Wu ZZ et al. (2011) J Cell Physiol 226, 2415–28

Smeenk L et al. (2011) PLoS One 6, e17574

Grison A et al. (2011) Proc Natl Acad Sci U S A 108, 17979–84

Gully CP et al. (2012) Proc Natl Acad Sci U S A 109, E1513–22

Wang H et al. (2012) DNA Repair (Amst) 11, 146–56

Xu S et al. (2013) Cell Res 23, 423–35

Chan C et al. (2013) Mol Cell Biol 33, 485–97

Thakur BK et al. (2013) Int J Cancer 132, 766–74

Yang Y et al. (2013) J Biol Chem 288, 529–39

Zhang Y et al. (2001) J Biol Chem 276, 20913–23

Romanelli A et al. (2002) J Biol Chem 277, 40281–9

Shi Y et al. (2002) J Biol Chem 277, 15712–20

Lehman JA et al. (2003) J Biol Chem 278, 28130–8

Le XF et al. (2003) Oncogene 22, 484–97

Gomez-Cambronero J and (2003) FEBS Lett 550, 94–100

Cammalleri M et al. (2003) Proc Natl Acad Sci U S A 100, 14368–73

Qiu Q et al. (2004) Mol Hum Reprod 10, 677–84

Strömberg T et al. (2004) Blood 103, 3138–47

Lisse TS et al. (2011) FASEB J 25, 937–47

Ali, S.M. and Sabatini, D.M. (2005) J Biol Chem 280, 19445-8.

Saitoh, M. et al. (2002) J Biol Chem 277, 20104-12.

Jung, C.H. et al. (2009) Mol Biol Cell 20, 1992-2003.

Zhang Y et al. (2001) J Biol Chem 276, 20913–23

Romanelli A et al. (2002) J Biol Chem 277, 40281–9

Shi Y et al. (2002) J Biol Chem 277, 15712–20

Lehman JA et al. (2003) J Biol Chem 278, 28130–8

Le XF et al. (2003) Oncogene 22, 484–97

Gomez-Cambronero J and (2003) FEBS Lett 550, 94–100

Cammalleri M et al. (2003) Proc Natl Acad Sci U S A 100, 14368–73

Qiu Q et al. (2004) Mol Hum Reprod 10, 677–84

Strömberg T et al. (2004) Blood 103, 3138–47

Lisse TS et al. (2011) FASEB J 25, 937–47

Ali, S.M. and Sabatini, D.M. (2005) J Biol Chem 280, 19445-8.

Saitoh, M. et al. (2002) J Biol Chem 277, 20104-12.

Jung, C.H. et al. (2009) Mol Biol Cell 20, 1992-2003.

Zhang Y et al. (2001) J Biol Chem 276, 20913–23

Romanelli A et al. (2002) J Biol Chem 277, 40281–9

Shi Y et al. (2002) J Biol Chem 277, 15712–20

Lehman JA et al. (2003) J Biol Chem 278, 28130–8

Le XF et al. (2003) Oncogene 22, 484–97

Gomez-Cambronero J and (2003) FEBS Lett 550, 94–100

Cammalleri M et al. (2003) Proc Natl Acad Sci U S A 100, 14368–73

Qiu Q et al. (2004) Mol Hum Reprod 10, 677–84

Strömberg T et al. (2004) Blood 103, 3138–47

Lisse TS et al. (2011) FASEB J 25, 937–47

Ali, S.M. and Sabatini, D.M. (2005) J Biol Chem 280, 19445-8.

Saitoh, M. et al. (2002) J Biol Chem 277, 20104-12.

Jung, C.H. et al. (2009) Mol Biol Cell 20, 1992-2003.

Dimberg A et al. (2000) Blood 96, 2870–8

Mowen KA et al. (2001) Cell 104, 731–41

Zhu W et al. (2002) J Biol Chem 277, 35787–90

Chen Q et al. (2002) Immunology 107, 199–208

Nair JS et al. (2002) Proc Natl Acad Sci U S A 99, 5971–6

DeVries TA et al. (2004) J Biol Chem 279, 45603–12

Timofeeva OA et al. (2006) Oncogene 25, 7555–64

Liu X et al. (2008) J Immunol 181, 449–63

Vanhatupa S et al. (2008) Biochem J 409, 179–85

Roth A et al. (2010) Proc Natl Acad Sci U S A 107, 19502–7

Chen Z et al. (2011) Cell Signal 23, 1404–12

Antunes F et al. (2011) Mol Cell Biol 31, 3029–37

Grönholm J et al. (2012) BMC Biochem 13, 20

Dimberg A et al. (2000) Blood 96, 2870–8

Mowen KA et al. (2001) Cell 104, 731–41

Zhu W et al. (2002) J Biol Chem 277, 35787–90

Chen Q et al. (2002) Immunology 107, 199–208

Nair JS et al. (2002) Proc Natl Acad Sci U S A 99, 5971–6

DeVries TA et al. (2004) J Biol Chem 279, 45603–12

Timofeeva OA et al. (2006) Oncogene 25, 7555–64

Liu X et al. (2008) J Immunol 181, 449–63

Vanhatupa S et al. (2008) Biochem J 409, 179–85

Roth A et al. (2010) Proc Natl Acad Sci U S A 107, 19502–7

Chen Z et al. (2011) Cell Signal 23, 1404–12

Antunes F et al. (2011) Mol Cell Biol 31, 3029–37

Grönholm J et al. (2012) BMC Biochem 13, 20

Ng J and Cantrell D (1997) J Biol Chem 272, 24542–9

Schuringa JJ et al. (2000) Blood 95, 3765–70

Duarte RF and Frank DA (2000) Blood 96, 3422–30

Schuringa JJ et al. (2001) Oncogene 20, 5350–8

Deb A et al. (2001) EMBO J 20, 2487–96

Abe K et al. (2001) Oncogene 20, 3464–74

Kopantzev Y et al. (2002) Oncogene 21, 6791–800

Sun S and Steinberg BM (2002) J Gen Virol 83, 1651–8

Hwang JH et al. (2003) Mol Endocrinol 17, 1155–66

Lo RK et al. (2003) J Biol Chem 278, 52154–65

Liu H et al. (2003) Blood 102, 344–52

Lo RK and Wong YH (2004) Mol Pharmacol 65, 1427–39

Wang R et al. (2005) J Biol Chem 280, 11528–34

Yuan ZL et al. (2005) Science 307, 269–73

Ray S et al. (2005) Gastroenterology 129, 1616–32

Liu AM et al. (2006) J Biol Chem 281, 35812–25

Zhang X et al. (2007) Proc Natl Acad Sci U S A 104, 4060–4

Ohbayashi N et al. (2007) Biol Pharm Bull 30, 1860–4

Nadiminty N et al. (2007) Biochem Biophys Res Commun 359, 379–84

Duechting A et al. (2008) J Virol 82, 7942–52

Chen CL et al. (2008) Mol Cancer 7, 78

Hatziapostolou M et al. (2011) Cell 147, 1233–47

Sestito R et al. (2011) FASEB J 25, 916–27

Gupta M et al. (2012) Leukemia 26, 1356–64

Perl AE et al. (2012) Clin Cancer Res 18, 1716–25


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
PathScan® is a trademark of Cell Signaling Technology, Inc.
LumiGLO® is a registered trademark of Kirkegaard & Perry Laboratories.