News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.


Antibody Guarantee

CST Antibody Performance Guarantee


To get local purchase information on this product, click here


Find answers on our FAQs page.


Visit PhosphoSitePlus®

PTM information and tools available.


Show Hidden Content

YES! Alert me when the status of this product changes.

Product Usage Information

This product should be used in place of the secondary in the western blotting protocol.

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Product Description

This Cell Signaling Technology product is useful for the detection of IgG. Conjugation of horseradish peroxidase (HRP) to protein A is obtained by cross-linking the amino groups on protein A with the carbohydrate groups on HRP.

Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and therefore can be used for detection and/or purification of antibodies. Due to its vast array of applications, protein A is the method of choice for many preparative and analytical immunological assays (1).

1.  Goding, J.W. (1978) J Immunol Methods 20, 241-53.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
XP® is a trademark of Cell Signaling Technology, Inc.
Tween® is a registered trademark of ICI Americas, Inc.