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To Purchase # 12348S

12348S 1 Kit (4 x 40 µl)

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Product Usage Information

Protocols

2975: Immunofluorescence, Flow

3939: Flow

4323: Flow

9174: Flow

Product Description

The Stat Family Alexa Fluor® 488 Conjugated Antibody Sampler Kit provides an economical means to study the activation status of members of the Stat family of proteins without the need for a fluorescent secondary antibody.


Specificity / Sensitivity

Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of Stat1 only when phosphorylated at Tyr701. This antibody detects phosphorylated Tyr701 of p91 Stat1 and also the p84 splice variant. It does not cross-react with the corresponding phospho-tyrosines of other Stat proteins. Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the corresponding phospho-tyrosines of other Stat proteins. Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699.


Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr701 of human Stat1 protein, Tyr705 of mouse Stat3 protein, or Tyr694 of human Stat5a protein.

Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors, and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules, and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to interferon response element (IRE) and gamma interferon-activated sequence (GAS) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stat family members is determined in part by the tissue-specific expression of different cytokine receptors, Jak and Stat family members (2,3), and by the combinatorial coupling of various Stat members to different receptors. Stat1 is activated in response to a large number of ligands (4) and is essential for responsiveness to IFN-α and IFN-γ (5,6). Stat3 is an important signaling molecule for many cytokines and growth factor receptors (4) and is required for murine fetal development (7). Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (8,9).


1.  Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.

2.  Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.

3.  Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322.

4.  Caldenhoven, E. et al. (1996) J Biol Chem 271, 13221-7.

5.  Durbin, J.E. et al. (1996) Cell 84, 443-50.

6.  Darnell, J.E. et al. (1994) Science 264, 1415-21.

7.  Meraz, M.A. et al. (1996) Cell 84, 431-42.

8.  Gouilleux, F. et al. (1994) EMBO J 13, 4361-9.

9.  Wakao, H. et al. (1994) EMBO J 13, 2182-91.


Entrez-Gene Id 6772, 6774, 6776, 6777
Swiss-Prot Acc. P42224, P40763, P42229, P51692

Protein Specific References

Dimberg A et al. (2000) Blood 96, 2870–8

Mowen KA et al. (2001) Cell 104, 731–41

Zhu W et al. (2002) J Biol Chem 277, 35787–90

Chen Q et al. (2002) Immunology 107, 199–208

Nair JS et al. (2002) Proc Natl Acad Sci U S A 99, 5971–6

DeVries TA et al. (2004) J Biol Chem 279, 45603–12

Timofeeva OA et al. (2006) Oncogene 25, 7555–64

Liu X et al. (2008) J Immunol 181, 449–63

Vanhatupa S et al. (2008) Biochem J 409, 179–85

Roth A et al. (2010) Proc Natl Acad Sci U S A 107, 19502–7

Chen Z et al. (2011) Cell Signal 23, 1404–12

Antunes F et al. (2011) Mol Cell Biol 31, 3029–37

Grönholm J et al. (2012) BMC Biochem 13, 20

Ng J and Cantrell D (1997) J Biol Chem 272, 24542–9

Schuringa JJ et al. (2000) Blood 95, 3765–70

Duarte RF and Frank DA (2000) Blood 96, 3422–30

Schuringa JJ et al. (2001) Oncogene 20, 5350–8

Deb A et al. (2001) EMBO J 20, 2487–96

Abe K et al. (2001) Oncogene 20, 3464–74

Kopantzev Y et al. (2002) Oncogene 21, 6791–800

Sun S and Steinberg BM (2002) J Gen Virol 83, 1651–8

Hwang JH et al. (2003) Mol Endocrinol 17, 1155–66

Lo RK et al. (2003) J Biol Chem 278, 52154–65

Liu H et al. (2003) Blood 102, 344–52

Lo RK and Wong YH (2004) Mol Pharmacol 65, 1427–39

Wang R et al. (2005) J Biol Chem 280, 11528–34

Yuan ZL et al. (2005) Science 307, 269–73

Ray S et al. (2005) Gastroenterology 129, 1616–32

Liu AM et al. (2006) J Biol Chem 281, 35812–25

Zhang X et al. (2007) Proc Natl Acad Sci U S A 104, 4060–4

Ohbayashi N et al. (2007) Biol Pharm Bull 30, 1860–4

Nadiminty N et al. (2007) Biochem Biophys Res Commun 359, 379–84

Duechting A et al. (2008) J Virol 82, 7942–52

Chen CL et al. (2008) Mol Cancer 7, 78

Hatziapostolou M et al. (2011) Cell 147, 1233–47

Sestito R et al. (2011) FASEB J 25, 916–27

Gupta M et al. (2012) Leukemia 26, 1356–64

Fox EM et al. (2008) Mol Endocrinol 22, 1781–96


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.