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REACTIVITY SENSITIVITY MW (kDa) Isotype
37 Rabbit 

Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

GAPDH (14C10) Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total GAPDH protein. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.


Species predicted to react based on 100% sequence homology: Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH.

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluroescent analysis of human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated GAPDH (14C10) Rabbit mAb #2118.


Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).


1.  Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.

2.  Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.

3.  Zheng, L. et al. (2003) Cell 114, 255-66.

4.  Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.

5.  Wang, Q. et al. (2005) FASEB J. 19, 869-71.


Entrez-Gene Id 2597
Swiss-Prot Acc. P04406


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
U.S. Patent No. 5,675,063.