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REACTIVITY SENSITIVITY MW (kDa) Isotype
70 Rabbit 

Product Usage Information

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Specificity / Sensitivity

Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (Biotinylated) detects endogenous levels of ATF-2 only when phosphorylated at Thr71. This antibody does not cross-react with phosphorylated c-Jun, CREB or other transcription factors. It recognizes both Thr69/Thr71 dually phosphorylated ATF-2 and Thr71 singly phosphorylated ATF-2 equally well.


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2 protein.

Product Description

This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb #5112 reacts with human, mouse, rat and monkey phospho-ATF-2 protein. CST expects that Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (Biotinylated) will also recognize phospho-ATF-2 in these species.


The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).


1.  Abdel-Hafiz, H.A. et al. (1992) Mol Endocrinol 6, 2079-89.

2.  Gupta, S. et al. (1995) Science 267, 389-93.

3.  van Dam, H. et al. (1995) EMBO J 14, 1798-811.

4.  Livingstone, C. et al. (1995) EMBO J 14, 1785-97.


Entrez-Gene Id 1386
Swiss-Prot Acc. P15336


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.