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REACTIVITY SENSITIVITY MW (kDa) Isotype
125 Rabbit IgG

Product Usage Information

Application Dilutions
Flow Cytometry 1:50

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Specificity / Sensitivity

Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb (PE Conjugate) recognizes endogenous levels of Jak2 protein only when phosphorylated at Tyr1008. This antibody also reacts with Jak2 when dually phosphorylated at Tyr1007 and Tyr1008. Cross-reactivity was not observed with other Jak family members by western blot.


Species Reactivity: Human, Mouse
Species predicted to react based on 100% sequence homology: Rat, Monkey, Xenopus, Bovine, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1008 of human Jak2 protein.

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb #8082.


Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.


1.  Darnell, J.E. (1997) Science 277, 1630-5.

2.  Leonard, W.J. (1998) Annu. Rev. Immunol. 16, 293-322.

3.  VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.

4.  Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.

5.  Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.


Entrez-Gene Id 3717
Swiss-Prot Acc. O60674


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Jak antibodies produced under license (granting certain rights including those under U.S. Patent No. 5,658,791) from Chemicon International, Inc.