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BackDrop® Green Background Suppressor
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BackDrop® Green Background Suppressor #12388

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Immunofluorescent analysis of HeLa cells using MitoTracker® Green FM #9074 in normal culture media, with (left) or without (right) the addition of BackDrop® Green Background Suppressor.

Product Usage Information

To eliminate green background fluorescence and improve live cell image quality, apply 2 drops of BackDrop® Green Background Suppressor per 1 mL of media just prior to imaging. Cells should be imaged within 1 hour of application, however longer treatment has not been shown to harm cells.

Storage

Store at room temperature. This product is stable for 12 months.

Product Description

BackDrop® Green Background Suppressor is a novel reagent designed to effectively suppress green background fluorescence and improve live cell image quality, eliminating the need for medium removal and potential cell loss. BackDrop® Green is supplied as a ready-to-use liquid in a convenient dropper bottle and is applied directly to live cell imaging samples.

Background

Background signal from media components can decrease signal-to-noise ratio and result in reduced assay sensitivity. The degree of background suppression with BackDrop® Green can vary depending on the assay, but can often be significant. BackDrop® Green has been shown to improve results when used in conjugation with MitoTracker® Green FM #9074, LysoTracker® Green DND-26 #8783, and ER-Tracker™ Green (BODIPY® FL Glibenclamide) #8787, particularly if phenol red-free media is not used in place of normal media prior to imaging.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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Inquiry Info.# 12388