News from the Bench
Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.
CST Antibody Performance Guarantee
To Purchase # 4083S
|$ 0. 00|
Find answers on our FAQs page.
PTM information and tools available.
Immunofluorescent analysis of HeLa cells using COX IV (3E11) Rabbit mAb #4850 (green), β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red) and DAPI (blue).Learn more about how we get our images
Gallery: DAPI #4083
DAPI is supplied as a lyophilized powder in 1 mg units. It can be used to examine cellular DNA in fluorescent microscopy applications.Molecular Formula:
- Free dye excitation maximum = 340 nm
- Free dye emission maximum = 488 nm
- DNA complex excitation maximum = 364 nm
- DNA complex emission maximum = 454 nm
This product is soluble up to 20 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, DAPI stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 to 0.1 μg/mL.Storage: Store desiccated and protected from light at 2-8°C. Lyophilized DAPI is stable for 2 years under these conditions. Soluble DAPI is stable for at least 2-3 weeks if protected from light.
4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) is a blue fluorescent DNA dye that targets double-stranded AT clusters in the DNA minor groove (1). One molecule of dye binds to each 3 base pairs of dsDNA and yields an approximate 20-fold fluorescent enhancement (2). The level of DAPI-DNA fluorescence is proportional to DNA content (3).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.