News from the Bench
Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.
Our U.S. Offices Will Be Closing Early
Our U.S. offices will be closing early on November 26th at 6:00 PM EST.
To Purchase # 8553S
Find answers on our FAQs page.
PTM information and tools available.
Gallery: PMSF #8553
Phenylmethanesulfonyl Fluoride (PMSF) is an inhibitor of serine proteases such as trypsin, chymotrypsin, thrombin, and papain. It is routinely added as a supplement to lysis buffers just prior to lysis, to prevent protease degradation. Cell Signaling Technology recommends adding PMSF at 1 mM to Cell Lysis Buffer (#9803) and RIPA Buffer (#9806). Molecular Formula: C7H7FO2S Molecular Weight: 174.19 CAS: 329-98-6Molecular Formula:
For a 200 mM (200X) stock solution, reconstitute the 34.84 mg of lyophilized PMSF supplied in the product tube in 1 mL isopropyl alcohol. PMSF is unstable in water and should be added to lysis buffers or other aqueous solutions just prior to use. Typically, a final concentration of 1 mM provides sufficient protease protection. Store lyophilized at RT for 12 months protected from direct light. Once in solution, PMSF can be stored at -20ºC for up to 3 months, protected from light.Storage: Store at Room Temperature
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.