News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.

Subscribe

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Thanksgiving. We will reopen on Monday, December 1st.

Thank you for your patience.

To Purchase # 8783S

8783S 500 µl (10 x 50 ul) $199.00
$ 0. 00

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Image
Image
Image

Immunofluorescence (Immunocytochemistry)

Confocal immunofluorescent analysis of live HeLa cells, untreated (left) or chloroquine-treated (50 μM, 4 hr; right), using LysoTracker® Green DND-26 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Learn more about how we get our images
Image
Image
Image

Product Description

LysoTracker® Green DND-26 is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 504 nm, Emission: 511 nm, Molecular Weight 398.69 g/mol


Product Usage Information

Each vial of LysoTracker® Green DND-26 contains 50 μl of solution at a 1 mM concentration in DMSO. Dilute 1:20,000 directly into normal growth media for a working concentration of 50 nM and analyze immediately. Cells must be imaged live; DO NOT FIX!

To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging. BackDrop® Green Background Suppressor #12388 can also be added to reduce background fluorescence.


Storage: Store at -20°C dessicated and protected from light. This reagent is stable for 6 months. Seal vial tightly after use to refreeze. Avoid excessive freeze-thaw cycles.

LysoTracker® Green DND-26 is a cell permeable green dye that stains acidic compartments (lysosomes) in live cells.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
BackDrop® is a registered trademark of Molecular Probes, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
LysoTracker® is a registered trademark of Molecular Probes, Inc.