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ProLong® Gold Antifade Reagent #9071
Confocal immunofluorescent analysis of HeLa cells using β-Actin (8H10D10) Mouse mAb #3700 (red) and COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).Learn more about how we get our images
Confocal immunofluorescent analysis of COS-7 cells, mounted in either glycerol (upper panels) or ProLong® Gold Antifade Reagent (lower panels), using β-Actin (8H10D10) Mouse mAb #3700 and Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4409 (red) (0, 3.3, or 10 min of imaging).Learn more about how we get our images
Gallery: ProLong® Gold Antifade Reagent #9071
Storage: ProLong® Gold Antifade Reagent is stable for 6 months when stored, protected from light, at or below -20ºC.
Following immunostaining, cover samples with ProLong® Gold Antifade Reagent and coverslip.
To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish.
Proceed with imaging.Storage: Store at -20°C. ProLong® Gold Antifade Reagent is stable for 6 months when stored, protected from light, at or below -20°C.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc. Alexa Fluor® is a registered trademark of Life Technologies Corporation. DRAQ5® is a registered trademark of Biostatus Limited.