News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.

Subscribe

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Thanksgiving. We will reopen on Monday, December 1st.

Thank you for your patience.

To Purchase # 9802S

9802S 15 ml

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Description

Kinase Buffer can be used to assay protein kinase activity.


Product Usage Information

1. If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. For longer periods of time, buffer should be stored at –20°C. Aliquotting of 10X buffer is recommended if many small experiments are to be performed.

2. Thaw 10X buffer at 24-30°C, mixing end-over-end.

3. Dilute the 10X buffer to the desired concentration using ddH2O.


Solutions and Reagents:

1X Kinase Buffer

25 mM Tris-HCl (pH 7.5), 5 mM beta-glycerophosphate, 2 mM dithiothreitol (DTT), 0.1 mM Na3VO4, 10 mM MgCl2.

Note: Kinase Buffer must be supplemented with ATP (#9804).


Storage: Store at -20C. For short term storage (1-2 weeks), Kinase Buffer can be stored at 4°C.

Product Specific References

Bisikirska, B.C. et al. (2012) Oncogene , .


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.