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12388S 2.5 ml $59.00
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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

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Immunofluorescence (Immunocytochemistry)

Immunofluorescent analysis of HeLa cells using MitoTracker® Green FM #9074 in normal culture media, with (left) or without (right) the addition of BackDrop® Green Background Suppressor.

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Product Description

BackDrop® Green Background Suppressor is a novel reagent designed to effectively suppress green background fluorescence and improve live cell image quality, eliminating the need for medium removal and potential cell loss. BackDrop® Green is supplied as a ready-to-use liquid in a convenient dropper bottle and is applied directly to live cell imaging samples.


Product Usage Information

To eliminate green background fluorescence and improve live cell image quality, apply 2 drops of BackDrop® Green Background Suppressor per 1 mL of media just prior to imaging. Cells should be imaged within 1 hour of application, however longer treatment has not been shown to harm cells.


Storage: Store at room temperature. This product is stable for 12 months.

Background signal from media components can decrease signal-to-noise ratio and result in reduced assay sensitivity. The degree of background suppression with BackDrop® Green can vary depending on the assay, but can often be significant. BackDrop® Green has been shown to improve results when used in conjugation with MitoTracker® Green FM #9074, LysoTracker® Green DND-26 #8783, and ER-Tracker™ Green (BODIPY® FL Glibenclamide) #8787, particularly if phenol red-free media is not used in place of normal media prior to imaging.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
BackDrop® is a registered trademark of Molecular Probes, Inc.
BODIPY is a registered trademark of Life Technologies Corporation.
LysoTracker® is a registered trademark of Molecular Probes, Inc.
MitoTracker is a registered trademark of Molecular Probes, Inc.

12388
BackDrop® Green Background Suppressor