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Product Includes Quantity (with Count) Solution Color
Phospho-Tyrosine Mouse mAb (P-Tyr-100) 9411 30 µl
HTScan® Tyrosine Kinase Buffer (4X) 9805 15 ml
DTT (1000X) 80 µl
ATP (10 mM) 9804 1 ml
PYK2 (Tyr402) Biotinylated Peptide 1315 1.25 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant human FGFR-1 kinase. It includes active FGFR-1 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula:

Peptide substrate, Biotin-Pyk2 (Tyr402): 2,166 Daltons. GST-FGFR-1 Kinase: 78 kDa.

Peptide Core Sequence:


Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors-FGF receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated and the receptor tyrosine kinase activated (2), resulting in further activation of various downstream signaling molecules and lysosomal degradation. FGFR has peptide substrate specificity similar to other receptor tyrosine kinase members, prefering acidic residues at the -1 to -4 positions and large hydrophobic amino acids at positions +1 and +3 (3).

1.  Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.

2.  Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.

3.  Songyang, Z. and Cantley, L.C. (1995) TIBS 20, 470-475.

Entrez-Gene Id 2260
Swiss-Prot Acc. P11362

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DELFIA is a registered trademark of PerkinElmer, Inc.