Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity (with Count) Solution Color
Phospho-PLK (Ser137) Antibody 30 µl
Kinase Buffer (10X) 9802 15 ml
ATP (10 mM) 9804 1 ml
PLK (Ser137) Biotinylated Peptide 1300 1.25 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant human Aurora A kinase. It includes active Aurora A kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-serine antibody for detection of the phosphorylated form of the substrate peptide.


Molecular Formula:

Biotin-peptide: 1,945 Daltons. GST-Aurora A Kinase: 72 kDa.


Peptide Core Sequence:

RRS*LL


Aurora kinases belong to a highly conserved family of mitotic serine/threonine kinases with three members identified among mammals: Aurora A, B, and C (1,2). Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Aurora kinase functional influences span from G2 phase to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning, and ingression (3). Aurora A is detected at the centrosomes, along mitotic spindle microtubules, and in the cytoplasm of mitotically proliferating cells. Aurora A protein levels are low during G1 and S phases and peak during the G2/M phase of the cell cycle. Phosphorylation of Aurora A at Thr288 in its catalytic domain increases kinase activity. Aurora A is involved in centrosome separation, maturation, and spindle assembly and stability. Expression of Aurora B protein also peaks during the G2/M phase of the cell cycle; Aurora B kinase activity peaks at the transition from metaphase to the end of mitosis. Aurora B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of both Aurora A and Aurora B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation (4,5); research investigators have observed overexpression of these kinases in a variety of human cancers (2,4). Aurora C localizes to the centrosome from anaphase to cytokinesis and both mRNA and protein levels peak during G2/M phase. Although typical Aurora C expression is limited to the testis, research studies report overexpression of Aurora C is detected in various cancer cell lines (6).


1.  Warner, S.L. et al. (2003) Mol Cancer Ther 2, 589-95.

2.  Katayama, H. et al. (2003) Cancer Metastasis Rev 22, 451-64.

3.  Andrews, P.D. et al. (2003) Curr Opin Cell Biol 15, 672-83.

4.  Pascreau, G. et al. (2003) Prog Cell Cycle Res 5, 369-74.

5.  Crosio, C. et al. (2002) Mol Cell Biol 22, 874-85.

6.  Kimura, M. et al. (1999) J Biol Chem 274, 7334-40.


Entrez-Gene Id 6790
Swiss-Prot Acc. O14965

Data Sheets & Documentation


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DELFIA® is a registered trademark of PerkinElmer, Inc.