Upstream / Downstream

Explore pathways related to this product.

Our U.S. Offices Will Be Closing Early

Our U.S. offices will be closing early on November 26th at 6:00 PM EST.

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity (with Count) Solution Color
Phospho-IκBα (Ser32) (14D4) Rabbit mAb 2859 30 µl
Kinase Buffer (10X) 9802 15 ml
ATP (10 mM) 9804 1 ml
IκBα (Ser32) Biotinylated Peptide 1146 1.25 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant human IKKbeta kinase. It includes active IKKbeta kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-serine antibody for detection of the phosphorylated form of the substrate peptide.


Molecular Formula:

Peptide substrate, Biotin-peptide: 2,085 Daltons. GST-IKKbeta Kinase: 87 kDa.


Peptide Core Sequence:

DS*GLDS*M


The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).


1.  Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.

2.  Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.

3.  Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.

4.  Brown, K. et al. (1995) Science 267, 1485-8.

5.  Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.

6.  Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.

7.  Chen, Z.J. et al. (1996) Cell 84, 853-62.

8.  Zandi, E. et al. (1997) Cell 91, 243-52.

9.  Karin, M. (1999) Oncogene 18, 6867-74.

10.  DiDonato, J.A. et al. (1997) Nature 388, 548-54.

11.  Mercurio, F. et al. (1997) Science 278, 860-6.

12.  Johnson, L.N. et al. (1996) Cell 85, 149-58.

13.  Delhase, M. et al. (1999) Science 284, 309-13.


Entrez-Gene Id 3551
Swiss-Prot Acc. O14920

Data Sheets & Documentation


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DELFIA® is a registered trademark of PerkinElmer, Inc.
U.S. Patent No. 5,675,063.