Upstream / Downstream

pathwayImage

Explore pathways related to this product.

Antibody Guarantee

CST Antibody Performance Guarantee

LEARN MORE  

To get local purchase information on this product, click here

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity (with Count) Solution Color
Phospho-Tyrosine Hydroxylase (Ser40) Antibody 2791 30 µl
Kinase Buffer (10X) 9802 15 ml
ATP (10 mM) 9804 1 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant human MAPKAPK-5 kinase. It includes active MAPKAPK-5 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-serine/threonine antibody for detection of the phosphorylated form of the substrate peptide.


Molecular Formula:

Peptide substrate, Biotin- Tyrosine Hydroxylase (Ser40): 2,326 Daltons. GST-MAPKAPK-5 Kinase: 84 kDa.


Peptide Core Sequence:

RRQS*LIE


MAPKAPK-5 belongs to the mitogen-activated protein kinase (MAPK) activated protein kinases (MK) subfamily that includes MAPKAPK-2/MK2 and MK3/3pK. The MK subfamily is part of a family of protein kinase subfamilies downstream of MAPK pathways and includes ribosomal S6 kinase (RSKs), mitogen and stress activated kinases (MSKs) and MAPK-interacting kinases (MNKs). All MKs are activated by MAPK pathways and mediate important processes (e.g. gene expression, cell cycle progression) and have been implicated in inflammation and cancer (1,2). MAPKAPK-5 shows binding to and activation by p38 MAPK and extracellular-regulated kinases (Erk) (3,4). MAPKAPK-5 was shown to be activated by Erk3 and act as a chaperone to Erk3 (5,6). While overexpressed MAPKAPK-5 shares similar substrates with MAPKAPK-2, such as HSP27 and glycogen synthase, recent work with MAPKAPK-5 knock-out mice indicates distinct substrates and functional properties (7).


1.  Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.

2.  Gaestel, M. (2006) Nat. Rev. Mol. Cell Biol. 7, 120-130.

3.  New, L. et al. (1998) EMBO J. 17, 3372-3384.

4.  Ni, H. et al. (1998) Biochem. Biophys. Res. Commun. 243, 492-496.

5.  Schumacher, S. et al. (2004) EMBO J. 23, 4770-4779.

6.  Seternes, O.M. et al. (2004) EMBO J. 23, 4780-4791.

7.  Shi, Y. et al. (2003) Mol. Cell Biol. 23, 7732-7741.


Entrez-Gene Id 8550
Swiss-Prot Acc. Q8IW41

Data Sheets & Documentation


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DELFIA® is a registered trademark of PerkinElmer, Inc.