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Product Includes Quantity (with Count) Solution Color
Phospho-Tyrosine Hydroxylase (Ser40) Antibody 2791 30 µl
Kinase Buffer (10X) 9802 15 ml
ATP (10 mM) 9804 1 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant human MAPKAPK-5 kinase. It includes active MAPKAPK-5 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-serine/threonine antibody for detection of the phosphorylated form of the substrate peptide.


Molecular Formula:

Peptide substrate, Biotin- Tyrosine Hydroxylase (Ser40): 2,326 Daltons. GST-MAPKAPK-5 Kinase: 84 kDa.


Peptide Core Sequence:

RRQS*LIE


MAPKAPK-5 belongs to the mitogen-activated protein kinase (MAPK) activated protein kinases (MK) subfamily that includes MAPKAPK-2/MK2 and MK3/3pK. The MK subfamily is part of a family of protein kinase subfamilies downstream of MAPK pathways and includes ribosomal S6 kinase (RSKs), mitogen and stress activated kinases (MSKs) and MAPK-interacting kinases (MNKs). All MKs are activated by MAPK pathways and mediate important processes (e.g. gene expression, cell cycle progression) and have been implicated in inflammation and cancer (1,2). MAPKAPK-5 shows binding to and activation by p38 MAPK and extracellular-regulated kinases (Erk) (3,4). MAPKAPK-5 was shown to be activated by Erk3 and act as a chaperone to Erk3 (5,6). While overexpressed MAPKAPK-5 shares similar substrates with MAPKAPK-2, such as HSP27 and glycogen synthase, recent work with MAPKAPK-5 knock-out mice indicates distinct substrates and functional properties (7).


1.  Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.

2.  Gaestel, M. (2006) Nat. Rev. Mol. Cell Biol. 7, 120-130.

3.  New, L. et al. (1998) EMBO J. 17, 3372-3384.

4.  Ni, H. et al. (1998) Biochem. Biophys. Res. Commun. 243, 492-496.

5.  Schumacher, S. et al. (2004) EMBO J. 23, 4770-4779.

6.  Seternes, O.M. et al. (2004) EMBO J. 23, 4780-4791.

7.  Shi, Y. et al. (2003) Mol. Cell Biol. 23, 7732-7741.


Entrez-Gene Id 8550
Swiss-Prot Acc. Q8IW41

Data Sheets & Documentation


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DELFIA® is a registered trademark of PerkinElmer, Inc.