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Product Includes Quantity (with Count) Solution Color
Phospho-Tyrosine Mouse mAb (P-Tyr-100) 9411 30 µl
HTScan® Tyrosine Kinase Buffer (4X) 9805 15 ml
DTT (1000X) 80 µl
ATP (10 mM) 9804 1 ml
FLT3 (Tyr589) Biotinylated Peptide 1305 1.25 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant human FGFR-3 kinase. It includes active FGFR-3 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.


Molecular Formula:

Peptide substrate, Biotin-FLT3 (Tyr589): 1,945Daltons. GST-FGFR-3 Kinase: 82 kDa.


Peptide Core Sequence:

NEY*FY*V


Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).


1.  Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.

2.  Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8.

3.  Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.

4.  Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78.

5.  Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.


Entrez-Gene Id 2261
Swiss-Prot Acc. P22607

Data Sheets & Documentation


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DELFIA® is a registered trademark of PerkinElmer, Inc.