Upstream / Downstream

Explore pathways related to this product.

Our U.S. Offices Are Closed

Our U.S. offices are closed in observance of Thanksgiving. We will reopen on Monday, December 1st.

Thank you for your patience.

Questions?

Find answers on our FAQs page.

ANSWERS  

Visit PhosphoSitePlus®

PTM information and tools available.

LEARN MORE

Product Includes Quantity (with Count) Solution Color
Phospho-Tyrosine Mouse mAb (P-Tyr-100) 9411 30 µl
HTScan® Tyrosine Kinase Buffer (4X) 9805 15 ml
DTT (1000X) 80 µl
ATP (10 mM) 9804 1 ml
PYK2 (Tyr402) Biotinylated Peptide 1315 1.25 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant human FGFR-4 kinase. It includes active FGFR-4 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.


Molecular Formula:

Peptide substrate, Biotin-peptide: 2,166 Daltons. GST-FGFR-4 Kinase: 74 kDa.


Peptide Core Sequence:

DIY*AE


Fibroblast growth factors are a family of broad-spectrum growth factors influencing a plethora of cellular activities. The interaction of at least 23 ligands, 4 receptors and multiple coreceptors provides a dramatic complexity to a signaling system capable of effecting a multitude of responses (1,2). Basic fibroblast growth factor (bFGF or FGF2), initially identified as a mitogen with prominent angiogenic properties, is now recognized as a multifunctional growth factor (3). It is clear that bFGF produces its biological effects in target cells by signaling through cell-surface FGF receptors. bFGF binds to all four FGF receptors. Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic downstream target proteins, including FRS-2, PLC and Crk (4,5). The FGF signaling pathway appears to play a significant role not only in normal cell growth regulation but also in tumor development and progression (6).

Acidic FGF (aFGF or FGF1) is another extensively investigated protein of the FGF family. aFGF shares 55% DNA sequence homology with bFGF. These two growth factors are ubiquitously expressed and exhibit a wide spectrum of similiar biological activities with quantitative differences likely due to variation in receptor affinity or binding (7).


1.  Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.

2.  Bansal, R. (2002) Dev. Neurosci. 24, 35-46.

3.  Morrison, R.S. et al. (1994) J. Neurooncol. 18, 207-216.

4.  Kouhara, H. et al. (1997) Cell 89, 693-702.

5.  Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78.

6.  Bikfalvi, A. (1995) Eur. J. Cancer 31A, 1101-1104.

7.  Ledoux, D. et al. (1992) Prog. Growth Factor Res. 4, 107-120.


Entrez-Gene Id 2264
Swiss-Prot Acc. P22455

Data Sheets & Documentation


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DELFIA® is a registered trademark of PerkinElmer, Inc.