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Product Includes Quantity (with Count) Solution Color
Phospho-Tyrosine Mouse mAb (P-Tyr-100) 9411 30 µl
HTScan® Tyrosine Kinase Buffer (4X) 9805 15 ml
DTT (1000X) 80 µl
Gastrin Precursor (Tyr87) Biotinylated Peptide 1310 1.25 ml
ATP (10 mM) 9804 1 ml

Product Description

The kit provides a means of performing kinase activity assays with recombinant human FLT3 kinase. It includes active FLT3 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide


Molecular Formula:

Peptide substrate, Biotin-peptide: 2,853 Daltons. GST-FLT3 Kinase: 82 kDa.


Peptide Core Sequence:

EAY*GW


FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).


1.  Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev 9, 37-48.

2.  Naoe, T. et al. (2001) Cancer Chemother Pharmacol 48 Suppl 1, S27-30.

3.  Namikawa, R. et al. (1996) Stem Cells 14, 388-95.

4.  Beslu, N. et al. (1996) J Biol Chem 271, 20075-81.

5.  Zhang, S. and Broxmeyer, H.E. (2000) Biochem Biophys Res Commun 277, 195-9.

6.  Zhang, S. et al. (1999) J Leukoc Biol 65, 372-80.

7.  Mizuki, M. et al. (2000) Blood 96, 3907-14.


Entrez-Gene Id 2322
Swiss-Prot Acc. P36888

Data Sheets & Documentation


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
DELFIA® is a registered trademark of PerkinElmer, Inc.